The NOG Mouse Portfolio

THE NOG MOUSE PORTFOLIO OVERVIEW

The CIEA NOG mouse® is a versatile and widely used super immunodeficient model with broad global adoption by researchers in both academia and industry. The NOG mouse is the model of choice for xenografts, particularly difficult to engraft cell lines (CDX) and patient-derived xenografts (PDX), as well as immune system humanization. The NOG mouse is the base of a flexible platform which includes a variety of second generation models which have specific utility as well as several different types of humanized models.

NOG MOUSE HIGHLIGHTS

A super immune deficient mouse with unparalleled potential for engraftment of human cells and tissues.

Severly immunodeficient mouse

Lacks T, B, NK cells
Reduced complement activity
Dysfunctional macrophages and dendritic cells
Deficiencies in immune signaling, including impaired cytokine production

Versatile and widely used

Superior host for xenogeneic cells and tissues

CDX and PDX
Immune system humanization
NOD SIRPα allele promotes enhanced engraftment of human hematopoietic lineages

HIGH LEVEL COMPARISON OF IMMUNODEFICIENT MICE

STRAIN	IMMUNE DEFICIENCIES	OTHER CONSIDERATIONS	KEY APPLICATIONS
NUDE MICE Foxn1^nu mutation, various inbred and outbred backgrounds		Immune system humanization Relatively normal radiation sensitivity	Outbred nudes are excellent general purpose xenograft hosts, but are not recommended for difficult-to-engraft cell lines or patient-derived xenografts Appropriate for efficacy studies involving tumor irradiation Inbred nudes may be useful for adoptive transfer studies Cannot be used as hosts for immune system humanization
SCID MICE Prkdc^scid mutation, various inbred and outbred backgrounds		Radiation sensitive Can become "leaky" and develop functional T and B cells, including antibody production	Scid mice are commonly used for xenografts of cell lines which do not grow well in nude mice Not preferred as hosts for current humanization protocols
NOD SCID MICE^* Prkdc^scid mutation on inbred NOD strain background	Reduced NK cell activity. Defective dendritic cells and macrophages. Reduced complement activity.	Radiation sensitive Can become "leaky" and develop functional T and B cells, including antibody production Significantly shortened lifespan due to development of thymic lymphomas NOD SIRPα allele promotes superior engraftment of human hematopoietic lineages	NOD scid mice are commonly used as xenograft hosts for difficult-to-engraft cell lines and patient-derived xenografts Preferred as a humanization host for certain experiments in which presence of gut-associated lymph tissue (GALT) may be relevant, but does not engraft as efficiently as NOG Not appropriate for longer-term experiments due to limited lifespan
CIEA NOG mouse® Prkdc^scid mutation and Il2rg knockout on inbred NOD strain background	Reduced complement activity, dysfunctional macrophages and dendritic cells, deficiencies in immune signaling, including cytokine production.	Radiation sensitive Not leaky Normal lifespan with appropriate housing and husbandry NOD SIRPα allele promotes superior engraftment of human hematopoietic lineages More sensitive than less immunodeficient models to opportunistic infections. Requires special housing and husbandry	NOG mice are superior xenograft hosts for difficult-to-engraft cell lines and patient-derived xenografts Superior host for immune system humanization, including engraftment of human peripheral blood mononuclear cell (PBMCs), hematopoietic stem cells (HSCs), and co-engraftment with human tumors Many 2nd generation NOG models available to support engraftment and differentiation of specific human immune cell types and PDX

*Not available from Taconic Biosciences via live production

Platform Built on the NOG mouse

2^ND GEN NOG MODELS		HUMANIZED IMMUNE SYSTEM MODELS
B2m-NOG NOG-EXL TK-NOG	hIL-2 NOG hIL-6 NOG hIL-15 NOG	huNOG huNOG-EXL SA huNOG-EXL EA	huPBMC-NOG huPBMC-B2m-NOG

NOG MOUSE PORTFOLIO STRAINS

The NOG Mouse Portfolio offers a series of super immunodeficient NOG strains expressing different human cytokines which may better support the engraftment of certain tumor types and which are used as hosts for a variety of humanized immune system (HIS) models.

MODEL	DESCRIPTION	XENOGRAFT APPLICATIONS
CIEA NOG mouse®	A super immune deficient mouse with unparalleled potential for engraftment of human cells and tissues.	Difficult to engraft cell lines and patient-derived tumors; Immune system humanization, including co-engraftment of human tumors
NOG-EXL	NOG expressing human GM-CSF and IL-3	Studies involving human myeloid cells; host for acute myeloid leukemia (AML) PDX
hIL-2 NOG	NOG expressing human IL-2	Research involving human T cells, CAR-T cell efficacy studies, tumor infiltrating lymphocytes (TILs)
hIL-6 NOG	NOG expressing human IL-6	Studies involving human monocytes and macrophages, including tumor-associated macrophages (TAMs); host for multiple myeloma (MM) PDX
hIL-15 NOG	NOG expressing human IL-15	Studies involving human NK cells, including efficacy studies with antibody-dependent cellular cytotoxicity (ADCC) mechanisms
B2m-NOG	NOG mouse lacking B2m gene	Can successfully engraft human PBMCs and tumors. Compared to NOG, B2m-NOG mice have markedly delayed graft vs. host disease (GvHD) onset after human PBMC engraftment, providing an expanded study window (8+ weeks), vastly increasing the utility of the PBMC model for immuno-oncology experiments.

Humanized Immune System Models

Humanized immune system models are widely applied in preclinical immuno-oncology research. Selection of the best strain starts from determination of which human cell types are of interest.

Humanized

Human tumor with human immune cells
Limitations on immune cell engraftment
Potential for GvHD
Species mismatch between tumor/immune cells and stroma

Complex experiments require complex models.

Let us help you pick the right one.

Easiest Access to Commercial Humanized Mice

Access means researchers can start projects sooner, with immediate, off the shelf inventory, providing models within days and not months. Additionally, Taconic's humanized immune system mice, including second generation NOG strains, are distributed with no additional licensing requirements under clear for profit terms of use and nonprofit terms of use. Taconic Biosciences is the only rodent model provider empowering customers to obtain models without an MTA or commercial use license. These simple terms facilitate use in drug discovery, including in contract research studies.

CUSTOM SOLUTIONS FOR ONCOLOGY AND IMMUNO-ONCOLOGY RESEARCH

Taconic Biosciences' globally-recognized scientific project managers support your custom model design project from design to delivery. Each custom model design project develops along an agile framework, letting you reassess and retarget your model fluidly as new data becomes available. You can pause or postpone projects at any time, or even "back up" project milestones via cryopreservation.