Our Flow Cytometry Capabilities
At Taconic Biosciences, we offer advanced flow cytometry services utilizing both conventional and spectral instrumentation. This ensures we can meet a wide range of analytical needs for your research.
Flow Cytometry Services
The versatility of Flow Cytometry makes it an indispensable tool across immunology, oncology, and drug development research. Flow Cytometry enables tailored studies of cell signaling, immune response, and biomarker screening.
Launching Now: Profiling Immune Cells
We are excited to announce the immediate availability of our new immunophenotyping service, for the use of fully optimized immune panels for both humanized immune system and standard murine samples.
Key Applications
Our flow cytometry services support a variety of applications, including:
Immunophenotyping: Comprehensive analysis of immune cell populations.
Spectral & Conventional: Access to both spectral and conventional flow cytometry instrumentation.
Biomarker Analysis: Identification and quantification of cellular markers
Profile a Taconic Model Prior to Purchase: Gain valuable insights into the immune profiles of our animal models before your study begins.
Send Us Your Samples: Submit your samples in a stability buffer for expert analysis.
What You Should Know:
Following the initial launch of our optimized immunophenotyping panels, we are actively working to expand our flow cytometry offerings. This includes the development of custom panel design services applicable to both our custom model generation (CMGS) and colony management services (CMS).
Have Questions About Flow Cytometry?
Learn how flow cytometry can advance your work. Reach out to our team of scientific experts to discuss your specific research needs.
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Easy Access to Humanized Immune System Mice
The most robust and translatable rodent models available for immuno-oncology research, designed to recapitulate important aspects of the human immune system. Start projects sooner with off-the-shelf inventory available within days, not months.
Why Choose Taconic as a Flow Cytometry Provider?
Taconic Biosciences specializes in providing comprehensive Flow Cytometry services tailored to meet the needs of researchers and scientists working with our models:
- Expertise in HIS Mice: With extensive experience in creating and immunophenotyping Humanized Immune System mice and their base strains, our deep understanding and expertise ensure high-quality and reliable results.
- Enhanced Data and Detail: We are committed to meeting these needs of researchers by offering advanced Flow Cytometry services as part of our broader protein analysis solutions.
- Convenience and Integration: By conducting Flow Cytometry analysis in-house, we provide a seamless and convenient experience for researchers, allowing for efficient project management.
- Design to Data—Comprehensive Solutions: With our comprehensive Colony Management and Custom Model Generation Solutions, our integrated approach ensures that you receive the most comprehensive and effective solutions for your research needs.
What is Flow Cytometry?
Flow cytometry enables researchers to label proteins of interest using fluorophore-conjugated antibodies and other fluorochromes. Much like immunohistochemistry, which produces two- or three-dimensional images of tissue, flow cytometry allows researchers to assess cell-associated protein expression. This can be used to identify the frequency and numbers of a cell type within a sample or levels of expression of a protein of interest. Flow cytometry experiments have been used extensively in the preclinical to clinical pipeline allowing researchers to assess the immune response to therapeutics by evaluating the cellular phenotype before and during a course of treatment.
How Can Researchers Use Flow Cytometry in Their Studies?
Flow cytometry can be used to analyze a variety of different sample types for both in vivo and ex vivo studies. In a flow cytometry workflow, tissue, blood, or other sources of cells are isolated and processed into single cell suspensions containing the cells of interest. The cells are then labeled with cell viability dyes or fluorescent antibodies. Samples can then be analyzed live or fixed, allowing for later analysis, on a flow cytometer. Using commercially available software including FlowJo or FCS Express, cells can be measured for size (forward scatter), granularity (side scatter), cell viability, and cell surface or intracellular protein expression.
How Does Taconic Help Researchers Utilize Flow Cytometry?
Taconic’s experts can help discuss a wide range of topics related to flow cytometry and humanized immune system mice including: design of flow cytometry panels (including assessment of the myeloid compartment), important considerations for blood sampling during humanized mouse experiments, chimerism calculation methods, application of spectral flow cytometry to humanized mouse analysis, and the types of data provided with shipment of Taconic humanized immune system mice.
Effective mouse models that recapitulate human disease are key in advancing preclinical research. Severely immunodeficient mice as well as advanced humanized models have enabled a deeper understanding of disease mechanisms and accelerated the development of novel therapeutics that have a greater chance of success in clinical trials. Humanized models are generated from the myeloablation and subsequent hematopoietic stem cell (HSC) or peripheral blood mononuclear cells (PBMC) engraftment of a severely immunodeficient mouse. Variability between mouse models, along with other factors, contributes to a differing amount of engrafted immune cells in the resulting model. In order to fully understand the immune cell composition of a humanized mouse, it’s necessary to test the blood and tissues of interest and characterize the cell types present. Utilizing flow cytometry on these samples enables:
- Quality control of successful tumor engraftment in humanized models
- Randomization of animals before study conception
- Monitoring of immune cell reconstitution
- Characterizing cell populations, including cell proliferation and activation
- Endpoint analysis
- Tumor-infiltrating lymphocyte analysis
Featured Flow Cytometry Webinars
Additional Resources
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Download Our Immune Panel Data Pack
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Header Image: ZEISS Microscopy from Germany, CC BY 2.0, via Wikimedia Commons
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