B2m-NOG

Origin:

The CIEA NOG mouse® was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The Prkdc^scid mutation was identified by Mel Bosma of the Fox Chase Cancer Center in a C.B-17 congenic mouse population. This mutation was backcrossed onto the NOD/ShiJic strain at CIEA for at least eight generations. The Il2rg targeted mutation was developed by Dr. Kazuo Sugamura of Tohoku University by targeting the gene in ES cells derived from a 129 strain. Targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeras were backcrossed onto the C57BL/6JJic background for at least eight generations. The CIEA NOG mouse® was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc^scid line for a total of eight generations. The B2m gene was knocked out via CRISPR-mediated knock-in of a nonsense mutation into exon 1. This mutation was made directly in the NOG strain.

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Initial Publication:

Verma, B.; Ruggeri, B.; Dubé, P.; Volden, P.; Wesa, A. Unique Immunodeficient Murine Host Strains Impact Expansion and Engraftment of T cells in PBMC Humanized Mice. New York Academy of Sciences’ Frontiers in Cancer Immunotherapy. May 14-15, 2019; New York, NY.

Animal Diet:

• Japanese Patent No. 3,753,321
• US Patent No. 7,145,055; 5,464,764; 5,487,992; 5,627,059; 5,631,153; 5,789,215; 6,204,061; 6,653,113; 6,689,610
  EP Patent No. 1,338,198
• Japanese Trademark Reg. No. 4,823,423
• US Trademark Reg. No. 3,118,040
• EU Trademark Reg. No. 3,736,758

• This Model and biological materials derived from it may only be used for purchasers' internal research purposes in the Field, unless purchaser otherwise has rights from the Broad covering such use. "Field" means use as a research tool for research purposes, and expressly excludes any (a) clinical use, (b) human, veterinary, livestock or agricultural use, or (c) manufacture, distribution, sale, promotion, use or other exploitation as a testing service, therapeutic or diagnostic for humans or animals.
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B2m-NOG mice at 6-11 weeks of age were engrafted with varying doses of human PBMCs via tail vein injection. Engraftment was assessed weekly by flow cytometry of peripheral blood samples and is expressed as human CD3/mouse CD45.