- Also known as Alb-HSVtk-NOG and albumin-TK-NOG, super immunodeficient NOG mouse with transgenic expression of thymidine kinase under control of liver-restricted albumin promoter.
- Useful for humanizing liver via engraftment with human hepatocytes and/or humanizing immune system via engraftment of human immune cells/tissues or hematopoietic stem cells.
- Inducible ablation of murine hepatocytes by ganciclovir treatment enables stable, long-term engraftment of human hepatocytes.
- The premiere model for human liver toxicity, metabolism, and infectious disease.
- Other uses include safety and immunogenicity assessment.
- Please note the scientific nomenclature of this strain was updated in Sept 2021 to reflect most current allele designations.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
What our customers say:
“Experimental Pharmacology & Oncology (EPO) GmbH uses 1st and 2nd generation NOG mice provided by Taconic for its preclinical oncology service. These mice are included in novel concepts for the development of personalized treatment options and especially suited for humanization strategies. We are very satisfied with the quality of mice, the reliability of shipment and the high level of scientific support. Further, we very much appreciate the competent and always friendly communication.”Experimental Pharmacology & Oncology (EPO) GmbH
Origin:
The NOG mouse was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The Prkdc scid mutation was identified by Mel Bosma of the Fox Chase Cancer Center in a C.B-17 congenic mouse population. This mutation was backcrossed onto the NOD/ShiJic strain at CIEA for at least eight generations. The Il2rg targeted mutation was developed by Dr. Kazuo Sugamura of Tohoku University by targeting the gene in ES cells derived from a 129 strain. Targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeras were backcrossed onto the C57BL/6JJic background for at least eight generations. The NOG mouse was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc scid line for a total of eight generations. The HSVtk transgene was generated by CIEA via microinjection into NOD/Shi inbred mice. The transgenic construct contains the mouse albumin enhancer/promoter (mAlb En/Pro), the chimeric intron, HSVtk cDNA, and the 3'-UTR of the human growth hormone gene with a polyadenylation signal (hGH pA). There was a single site of transgene integration, and HSVtk mRNA was selectively expressed in the liver. The TK-NOD/Shi mice were backcrossed onto the NOG mouse at CIEA and imported into Taconic in 2014. Color:
Albino Species:
Mouse Initial Publication:
- Hasegawa M, Kawai K, Mitsui T, Taniguchi K, Monnai M, Wakui M, Ito M, Suematsu M, Peltz G, Nakamura M, Suemizu H. The reconstituted 'humanized liver' in TK-NOG mice is mature and functional. (2011) Biochem Biophys Res Commun. 18;405(3):405-10.
- Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, Heike T, Nakahata T. (2002) NOD/SCID/γγ
mouse: an excellent recipient mouse model for engraftment of human cells. Blood 100(9):3175-3182.
