- Super immunodeficient NOG mouse expressing human IL-15 cytokine
- Predominant differentiation of human NK cells following human HSC engraftment compared to the base NOG mouse, but survival time is limited
- Human NK cells developed in hIL-15 NOG mice following HSC engraftment express various NK receptors and produce both granzyme A and perforin upon stimulation
- Engraftment and expansion of human NK cells following engraftment with CD56+ NK cells derived from PBMCs
- May be useful for efficacy studies involving antibody therapeutics with antibody dependent cell cytotoxcity mechanism of action
- Applications in research involving cancer, infectious disease, immunology, regenerative medicine and human immune system engraftment
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Origin:
The CIEA NOG mouse® was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The Prkdc scid mutation was identified by Mel Bosma of the Fox Chase Cancer Center in a C.B-17 congenic mouse population. This mutation was backcrossed onto the NOD/ShiJic strain at CIEA for at least eight generations. The Il2rg targeted mutation was developed by Dr. Kazuo Sugamura of Tohoku University by targeting the gene in ES cells derived from a 129 strain. Portions of exons 7 and 8 were replaced with a neo cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeras were backcrossed onto the C57BL/6JJic background for at least eight generations. The CIEA NOG mouse® was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc scid line for a total of eight generations. The hIL-15 NOG mouse was developed by microinjecting a transgene consisting of a DNA fragment containing human IL-15 cDNA with a hIL-2 signal peptide, under the control of a CMV-promoter, into zygotes of NOD/ShiJic-Il2rg mice. Founders were backcrossed to NOG mice to establish the hIL-15 NOG line. Taconic received stock in 2014, and the line was derived through embryo transfer.
Availability:
Available now*
*Please note that advance order is encouraged for order of male mice. There is a minimal 4-week advance notice required on orders of male older than 3 weeks of age.
Color:
Albino
Species:
Mouse
Initial Publication:
Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, Heike T, Nakahata T. (2002) NOD/SCID/γ
mouse: an excellent recipient mouse model for engraftment of human cells.
Blood 100(9):3175-3182.
Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice.
Leuk Res 22(9):837-43.
*The hIL-15 NOG mouse has not been published yet. Initial publications above refer to the core NOG mouse.
