- The FcResolv™ NOG is a super immunodeficient NOG mouse that has a further reduction in residual murine immune activity via ablation of mouse Fc gamma receptors (FcγRs) to improve accuracy of results.
- Murine FcγRs can confound experiments in several ways, leading to false positive or negative results. Removing murine FcγRs provides improved accuracy for efficacy assessment of antibody-based therapies which include an Fc domain.
- Lacks all murine Fcγ receptors, including the FcγRI, IIB, III and IV types, along with the high affinity FcεRI receptor. The low affinity FcεRII receptor remains present.
- Can be used in a similar fashion to the NOG mouse, including for xenograft and immune system humanization experiments.
- FcResolv NOG mice support higher average levels of human cells following engraftment with HSCs compared to the base NOG mouse; human cell frequencies may vary somewhat between the two humanized models.
NOG Characteristics
- Amenable to immune system humanization via engraftment of human hematopoietic stem cells (HSCs).
- Immunodeficient model lacking mature T, B, and NK cells, with reduced complement activity and dysfunctional macrophages and dendritic cells.
- Excellent model for a variety of xenograft and human cell engraftment studies.
- Applications in research involving cancer, infectious disease, immunology, regenerative medicine, humanization, autoimmune disease, immunotherapy, vaccine, GvHD/transplantation, hematopoiesis, inflammation/allergy, and safety assessment.
- The Il2rg gene is sex-linked.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Origin:
The FcResolv NOG mouse was developed by Takeshi Takahashi of the Central Institute for Experimental Animals (CIEA) in Japan. The Fcer1gtm1Rav mutation was developed in the laboratory of J.V. Ravetch, Sloan-Kettering Institute. The Fcgr2btm1Ttk mutation was developed in the laboratory of T. Takai of Okayama University. These two mutations were backcrossed onto the CIEA NOG mouse®. Taconic received stock in 2021, and the line was derived through embryo transfer. The mice are maintained by breeding females homozygous for the Fcer1gtm1Rav, Fcgr2btm1Ttk, Prkdcscid and Il2rgtm1Sug mutant alleles with males that are homozygous for the Fcer1gtm1Rav, Fcgr2btm1Ttk, Prkdcscid alleles and hemizygous for the Il2rgtm1Sug mutant allele.
Availability:
Available now*
*Please note that advanced order placement is encouraged for male mice. Ordering 4 or more weeks prior to the desired shipping date is recommended when requesting males older than 3-weeks of age. Color:
Albino Species:
Mouse Initial Publication:
- Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020 Oct 7;11:532684.
- Katano I, Hanazawa A, Otsuka I, Yamaguchi T, Mochizuki M, Kawai K, Ito R, Goto M, Kagawa T, Takahashi T. Development of a novel humanized mouse model for improved evaluation of in vivo anti-cancer effects of anti-PD-1 antibody. Sci Rep. 2021 Oct 26;11(1):21087.
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