- Carries a transgene consisting of fragments of the human HLA-A*2402 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-A24 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains.
- Expresses the chimeric HLA-A24 class I molecule on the surface of T and B cells.
- Represents the human HLA-A24 supertype.
- Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology and autoimmune disorders and syngeneic tumor studies utilizing C57BL/6-derived tumors.
- Permits identification of epitopes restricted to the HLA-A24 supertype.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background:
C57BL/6NTac Origin:
The HLA-A24 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the genomic clone of the human HLA-A*2402 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-K
b gene containing the α3, transmembrane and cytoplasmic domains, all under the control of the murine H2-K
b promoter. This transgene was microinjected into C57BL/6 zygotes. The resultant mice from Founder Line A24.01 were on an inbred C57BL/6 background. The line was maintained by breeding hemizygotes to wild type C57BL/6NTac mice for 15 generations. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived by breeding C57BL/6NTac wild type females to hemizygous males, and the colony is maintained through breeding of hemizygous males to wild type C57BL/6NTac females. The strain is considered fully congenic on C57BL/6NTac background. In the production colony, wild type C57BL/6NTac animals are bred to hemizygous males on the C57BL/6NTac background to produce hemizygous pups. In addition, another production colony is breeding BALB/cAnNTac females with hemizygous males on the C57BL/6NTac background to produce hemizygous CB6F1 pups, which are sold under
model #9663.
Color:
Black Species:
Mouse Initial Publication:
Depla E, Van der Aa A, Livingston BD, Crimi C, Allosery K, De Brabandere V, Krakover J, Murthy S, Huang M, Power S, Babé L, Dahlberg C, McKinney D, Sette A, Southwood S, Philip R, Newman MJ, Meheus L. (2008) Rational design of a multiepitope vaccine encoding T-lymphocyte epitopes for treatment of chronic hepatitis B virus infections.
J Virol 82(1):435-50.