Black 6 (B6NTac)

C57BL/6 Mice - B6 Inbred Mouse Strain - Taconic Biosciences
  • Live
  • Mouse
  • Male & Female Available
  • Black
  • Available for direct purchase by CRO
Model No.NomenclatureGenotype
B6-FC57BL/6NTac
B6-MC57BL/6NTac
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Overview

Nomenclature: C57BL/6NTac

Taconic's B6 Provides Superior Performance

One of the most used inbred mouse models, the C57BL/6 is used in nearly every research application, and it's commonly used as the genetic background for transgenic mouse models. It is also the preferred model for studying diet-induced obesity and the chronic experimental autoimmune encephalomyelitis model of multiple sclerosis.

  • Popular research applications include oncology, immunology, metabolic disease, addiction and toxicology
  • C57BL/6NTac is the preferred C57BL/6 substrain for generation of genetically engineered models, including by the Knockout Mouse Project (KOMP) and International Mouse Phenotyping Consortium (IMPC)
  • The Taconic B6 has superior reproductive performance compared to other B6 substrains: superovulated females yield a high number of embryos and males have higher sperm fertilization rates
  • The Taconic C57BL/6NTac has proven more amenable to the development of quality embryonic stem cells compared to other B6 substrains
  • Popular strain for syngeneic tumor experiments; with a suite of related strains on the same background for mechanistic studies including Rag2, Rag2/OT-I, Rag2/OT-II and many more
  • Historical data shows that hydrocephaly occurs in the C57BL/6 mouse in 0.05% of the population and may not be evident until the animal is more than two months of age.

Availability

Available at the Murine Pathogen Free™, Opportunist Free™, and Germ Free health standards. Order model #B6 for Murine Pathogen Free™ and Opportunist Free™. Order model #GF-B6 for Germ Free. Inquire about ordering mice positive or negative for Segmented Filamentous Bacteria (SFB).

Origin

C57BL/6 litters were received in 1991 at F151 from the NIH Animal Genetic Resource. Cesarean derived in 1991 at Taconic, a foundation colony is maintained in gnotobiotic isolators. Origin is as follows: to NIH in 1951 from Jax at F32; to Jax in 1948 from Hall.

Genetics

Species:Mouse
Strain Type:Inbred
Coat Color:Black
Coat Color Loci: a/a, B/B, C/C
Genetic Background:C57BL/6
Coat Color Loci: a/a, B/B, C/C

Other Genetic Notes:

  • Strain Profile: Acy1f, Aladb, Car2a, Cas1g, Cd5b, Cd72b, Cd8ab, Cd8b1b, Ce2a, Es1a, Es3a, Esda, Ggc1b, Gpi1b, Gus-sb, H6pd1b, Hbaa, Hbbs, Hc1, Idh1a, Mod1b, Mup1b, Pep3a, Pgm1a, Trfb
  • Immunology: H2b, Ptprcb, Thy1b, H2-T18b
  • Retinal Degeneration: Crb1Rd8

Applications & Therapeutic Areas

  • ADMET
  • Microbiome
  • Model Generation Solutions
  • Neuroscience
  • Oncology & Immuno-Oncology
  • Psychiatric Disorders
  • Transgenic Model Generation Support
  • Vaccine Research
  • Autoimmune Disease
  • Cardiovascular Disease
  • Diabetes
  • Immunology
  • Infectious Disease
  • Inflammation
  • Inflammatory Bowel Disease
  • Metabolic Disease

Transit, Housing & Welfare

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B6 males at 7+ weeks of age are packed as cagemates, without recombination of animals from different cages into a single Taconic Transit Cage (TTC). We recommend that customers do NOT recombine 7+ week old B6 males from different Taconic Transit Cages into the same cage at or after unpacking. 

Packing Practices

Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns.  When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.

Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age."  Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.

Animal Aging

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Special Diet Administration

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Surgical Models and Services

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Data

Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6NTac mice

Chart demonstrates disease incidence percentage based on 5 various groups
Representative data for Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6NTac provided by Biomodels. Download the full data package.
Historical data shows that hydrocephaly occurs in the C57BL/6 mouse in 0.05% of the population and may not be evident until the animal is more than two months of age.

C57BL/6NTac substrains carry the Crb1Rd8 mutation.Retinal degeneration 8, is a mild form of retinal degeneration that affects vision. This recessive mutation is a single base pair mutation in the Crb1 gene, Crb1Rd8 (Mehalow et al. 2003) The onset of the phenotype appears to be between 2 and 6 weeks of age (Pritchett-Corning, 2012).

C57BL/6J mice present a deletion (~17 Kbp) in the Nicotinamide Nucleotide Transhydrogenase (Nnt) gene. Nnt is a transmembrane protein that catalyzes the inter conversion of NADH and NADPH in the mitochondria. Studies suggest that the presence of Nnt is essential for mitochondrial defense against oxidative stress and for normal cellular metabolism (Huang et al. 2006). Taconic’s C57BL mice, including C57BL/6JBomTac, C57BL/6NTac and C57BL/10SgSnAiTac, or our B6D2F1 and B6129F1, do not carry the Nnt mutation which is present in C57BL/6J sub-strain.

Reproductive Data

  • Average litter size: 6-8
  • Mean # litters/female: 7
  • Mean age breeder retired: 10 months
  • Mean # days to litter: 22

Licensing

Terms of Sale and Use for Black 6 (B6NTac)

Germ Free Black 6 are sold subject to Taconic's Terms and Conditions for Taconic's Models, Products and Services and the following terms of use:

  • For-profit purchasers may not breed Germ Free Black 6 except to obtain embryos or fetuses for research use.
  • Non-profit purchasers may breed Germ Free Black 6 for use in projects at purchaser's institution and on behalf of non-profit researchers only.
  • Germ Free Black 6, progeny and materials derived from them will not be distributed to third parties.
Murine Pathogen Free™, Restricted Flora™, and Opportunist Free Black 6 mice are sold subject to Taconic's Terms and Conditions for Taconic's Models, Products and Services.

Pricing - USD

Opportunist Free (OF) Health Standard

B6 Female

B6-F

Age in WeeksQuantity 1 - 999
3 to 4$54.00
5 to 6$67.00
7 to 8$78.00
9 to 10$87.00

Specialized Inventory

B6-FLQuantity 1 - 199
Female With Litter$434.00

Specialized Inventory

B6-TPQuantity 1 - 199
Timed Pregnant Female$511.00

Specialized Inventory

B6-UPQuantity 1 - 199
Untimed Pregnant Female$355.00

*Untimed pregnant requests are filled with late gestation pregnant females. Pups may be delivered in transit.

Specialized Inventory

B6-RFQuantity 1 - 199
Retired Female Breeder$93.00

B6 Male

B6-M

Age in WeeksQuantity 1 - 999
3 to 4$54.00
5 to 6$67.00
7 to 8$78.00
9 to 10$87.00

Specialized Inventory

B6-RMQuantity 1 - 199
Retired Male Breeder$93.00

Murine Pathogen Free (MPF) Health Standard

B6 Female

B6-F

Age in WeeksQuantity 1 - 199Quantity 200 - 999
3 to 4$31.00$28.00
5 to 8$39.00$33.00
9 to 10$45.00$41.00
11 to 12$52.00$46.00

Specialized Inventory

B6-UPQuantity 1 - 199
Untimed Pregnant Female$247.00

*Untimed pregnant requests are filled with late gestation pregnant females. Pups may be delivered in transit.

Specialized Inventory

B6-FLQuantity 1 - 199
Female With Litter$325.00

Specialized Inventory

B6-TPQuantity 1 - 199
Timed Pregnant Female$347.00

Specialized Inventory

B6-RFQuantity 1 - 199
Retired Female Breeder$55.00

B6 Male

B6-M

Age in WeeksQuantity 1 - 199Quantity 1 - 999Quantity 200 - 999
3 to 4$31.00$28.00
5 to 8$39.00$33.00
9 to 10$45.00$41.00
11 to 12$59.00$46.00
13 to 14$70.00
15 to 16$80.00
17 to 18$91.00
19 to 20$102.00
21 to 22$113.00
23 to 24$123.00
25 to 26$134.00
27 to 31$156.00
32 to 36$177.00
37 to 40$199.00
41 to 44$220.00
45 to 48$241.00
49 to 52$262.00

Specialized Inventory

B6-RMQuantity 1 - 199
Retired Male Breeder$55.00

Germ Free (GF) Health Standard

B6 Female

B6-F

Age in WeeksQuantity 1 - 29Quantity 30 - 999
4$587.00$559.00
5$653.00$623.00
6$678.00$644.00
7$707.00$673.00
8$737.00$701.00
9$781.00$745.00
10$825.00$785.00

Specialized Inventory

B6-RFQuantity 1 - 199
Retired Female Breeder$1,112.00

Specialized Inventory

B6-NPQuantity 1 - 999
Non-Pregnant Retired Female Breeder$1,332.00

Specialized Inventory

B6-UPQuantity 1 - 199
Untimed Pregnant Female$4,225.00

*Untimed pregnant requests are filled with late gestation pregnant females. Pups may be delivered in transit.

B6 Male

B6-M

Age in WeeksQuantity 1 - 29Quantity 30 - 999
4$587.00$559.00
5$653.00$623.00
6$678.00$644.00
7$707.00$673.00
8$737.00$701.00
9$781.00$745.00
10$825.00$785.00

Specialized Inventory

B6-RMQuantity 1 - 199
Retired Male Breeder$1,112.00

Pricing - EUR

Opportunist Free (OF) Health Standard

B6 Female

B6-F

Age in WeeksQuantity 1 - 999
3 to 4€34,00
5 to 6€37,00
7 to 8€41,00
9 to 10€47,00

Specialized Inventory

B6-RFQuantity 1 - 199
Retired Female Breeder€52,00

B6 Male

B6-M

Age in WeeksQuantity 1 - 999
3 to 4€33,00
5 to 6€36,00
7 to 8€39,00
9 to 10€49,00

Specialized Inventory

B6-RMQuantity 1 - 199
Retired Male Breeder€57,00

Murine Pathogen Free (MPF) Health Standard

B6 Female

B6-F

Age in WeeksQuantity 1 - 199Quantity 200 - 999
3 to 4€21,00€20,00
5 to 6€23,00€21,00
7 to 8€25,00€23,00
9 to 10€29,00€27,00
11 to 12€36,00€33,00

Specialized Inventory

B6-FLQuantity 1 - 199
Female With Litter€192,00

Specialized Inventory

B6-TPQuantity 1 - 999
Timed Pregnant Female€276,00

Specialized Inventory

B6-RFQuantity 1 - 199
Retired Female Breeder€34,00

B6 Male

B6-M

Age in WeeksQuantity 1 - 199Quantity 200 - 999
3 to 4€21,00€20,00
5 to 6€23,00€21,00
7 to 8€25,00€23,00
9 to 10€29,00€27,00
11 to 12€36,00€33,00
13 to 14€45,00
15 to 16€55,00
17 to 18€61,00
19 to 20€69,00
21 to 22€80,00
23 to 24€86,00
25 to 26€94,00
27 to 31€109,00
32 to 36€122,00
37 to 40€136,00
41 to 44€147,00
45 to 48€162,00
49 to 52€175,00

Specialized Inventory

B6-RMQuantity 1 - 199
Retired Male Breeder€36,00

Germ Free (GF) Health Standard

B6 Female

B6-F

Age in WeeksQuantity 1 - 29Quantity 30 - 999
4€585,00€558,00
5€655,00€623,00
6€675,00€644,00
7€705,00€673,00
8€735,00€700,00
9€781,00€745,00
10€824,00€784,00

Specialized Inventory

B6-UPQuantity 1 - 199
Untimed Pregnant Female€3.137,00

*Untimed pregnant requests are filled with late gestation pregnant females. Pups may be delivered in transit.

B6 Male

B6-M

Age in WeeksQuantity 1 - 29Quantity 30 - 999
4€585,00€558,00
5€655,00€623,00
6€675,00€644,00
7€705,00€673,00
8€735,00€700,00
9€781,00€745,00
10€824,00€784,00

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