Rag2 - Model 601

Constitutive Knockout

Rag2 (Model 601) Constitutive Knockout Mouse Model
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BALB/c Background

  • Model #
  • Genotype
  • Nomenclature
  • 601-F
    ko/ko
    C.129S6(B6)-Rag2tm1Fwa N12
  • 601-M
    ko/ko
    C.129S6(B6)-Rag2tm1Fwa N12

  • Contains a disruption of the recombination activating gene 2 (Rag2)
  • Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
  • Useful for vaccine development, transplantation studies and hematopoiesis research.
  • Carries the H2d haplotype
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:

BALB/c Background

Origin:

The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts.
Taconic received stock from the Ellis Reinherz lab at the Dana Farber Cancer Institute in 1993 as heterozygotes on a mixed B6;129S6 background. The mice were embryo transfer derived and intercrossed to generate homozygotes. Backcrossing to BALB/cAnNTac began at Taconic in September 1993, sponsored by Dana Farber Cancer Institute and screened by S. Koyasu. Backcrossing to N12 was completed in March 1997. The colony is maintained by incrossing homozygous mice.


Genetics:

Wild type for Nnt mutation


Color:

Albino

Species:

Mouse

Initial Publication:

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.



Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.


Rag2 Physiological Data Summary (February 2005; N=10/group):

Parameter Units 601(M) Males 601(M) Females
Serum Chemistry Avg ± S.D.    
Calcium mg/dL 10.2 ± 0.3 11.1 ± 0.2
Phosphorous mg/dL 8.0 ± 1.6 9.5 ±1.2
Glucose mg/dL 132 ± 26 86 ± 17
Creatinine mg/dL 0.2 ± 0.0 0.1 ± 0
BUN mg/dL 22 ± 4 18 ± 2
Total Bilirubin mg/dL 0.1 ± 0.0 0.1 ± 0
ALK U/L 161 ± 13 105 ± 73
ALT U/L U/L 52 ± 13 40 ± 9
Total Protein g/dL 5.3 ± 1.6 5.4 ± 0.2
Blood Counts
Eryhtron      
Red Blood Cells X10^6/uL 10.278 ± 0.605 9.986 ± 0.329
Nucleated RBC X10^6/uL 0.0 0.0
Hemoglobin g/dL 16.8 ± 1.0 16.2 ± 0.6
Hematocrit % 50.3 ± 2.9 48.8 ± 1.5
MCV fL 49 ± 0 49 ± 1
MCH pg 16.3 ± 0.2 16.2 ± 0.2
MCHC % 33.4 ± 0.4 33.2 ± 0.3
Platelets      
Platelets X10^3/uL 1225 ± 217 1086 ± 112
Leukogram      
White Blood Cells x10^3/uL 2.26 ± 1.148 1.187 ± 0.679
Neutrophil x10^3/uL 1.772 ± 0.884 0.826 ± 0.484
Bands x10^3/uL 0.0 0.0
Lymphocyte x10^3/uL 0.427 ± 0.244 0.311 ± 0.174
Monocytes x10^3/uL 0.037 ± 0.040 0.020 ± 0.012
Eosinophil x10^3/uL 0.024 ± 0.014 0.030 ± 0.022
Basophils x10^3/uL 0.0 0.0
Organ Weights      
Body Weight (Fasted) g 23.16 ± 2.11 16.78 ± 0.99
Stomach g 0.225 ± 0.035 0.210 ± 0.027
Ileum g 0.853 ± 0.161 0.693 ± 0.111
Colon g 0.218 ± 0.049 0.195 ± 0.029
Lungs g 0.273 ± 0.041 0.227 ± 0.016
Heart g 0.218 ± 0.027 0.145 ± 0.019
Liver g 1.326 ± 0.168 0.944 ± 0.070
Spleen/Pancreas g 0.245 ± 0.048 0.169 ± 0.023
Kidney (L) g 0.240 ±0.024 0.140 ± 0.018
Kidney (R) g 0.242 ± 0.031 0.149 ± 0.012
Testes (L) g 0.119 ± 0.008 -
Testes (R) g 0.117 ± 0.012 -
Ovary (L) g - 0.030 ± 0.010
Ovary (R) g - 0.032 ± 0.017

Rag2 Urinalysis Data


Male - sampled 05/08/03

Animal ID# 01 02 03 04 05 06 07 08 09 10
Glucose (mg/dl) Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg.
Blood Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg.
pH 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 7.5
Protein (mg/dl) 30+ 100++ 30+ 100++ 30+ 2000++++ 300+++ 100++ 30+ Neg.
Specific Gravity 1.030 1.030 1.030 1.030 1.020 1.030 1.030 1.030 1.030 1.010

Female - sampled 05/07/03

Animal ID# 11 12 13 14 15 16 17 18 19 20
Glucose (g/dl) Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg.
Blood Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg. Neg.
pH 5.0 5.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0
Protein (mg/dl) Trace Trace Trace 30+ Trace Trace 30+ 30+ 30+ Trace
Specific Gravity 1.030 1.030 1.030 1.030 1.030 1.030 1.030 1.030 1.030 1.020

Phenotyping Procedures:

Animal Receipt and Maintenance

A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line 601(M) (C.129S6(B6)-Rag2tm1Fwa N12), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH #31M rodent diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1 -10, females 11- 20).

Urinalysis

All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions.

Clinical Chemistry and Hematology

A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood and slides were delivered to LabCorp (RTP, NC) for analysis. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually.

Necropsy and Organ Weights

All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. The pathologist’s summary and detailed histological descriptions follow.
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