Rag2 - Model RAGN12

Rag2 (Model RAGN12) Constitutive Knockout Mouse Model
  • Live
  • Mouse
  • Male & Female Available
  • Black
  • Available for direct purchase by CRO
Model No.NomenclatureGenotype
RAGN12-FB6.129S6-Rag2tm1Fwa N12ko/ko
RAGN12-MB6.129S6-Rag2tm1Fwa N12ko/ko
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Overview

Nomenclature: B6.129S6-Rag2tm1Fwa N12

 

  • Contains a disruption of the recombination activating gene 2 (Rag2)
  • Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes. Otherwise, the Rag2 mouse has apparently normal hematopoiesis
  • Useful for vaccine development, transplantation studies and hematopoiesis research. Through blastocyst complementation assays, can be useful in evaluating the function of specific genes in the differentiation of lymphocytes
  • Carries the H2b haplotype
  • Taconic recommends use of RAGN12 mice at the Murine Pathogen Free (MPF) for colitis studies involving adoptive T cell transfer. Use of RAGN12 mice at the Opportunist Free (OF) health standard should be considered based on facility-specific bioexclusion needs and other experimental considerations.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Origin

The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. This line originated from model RAG2-M on a 129S6/SvEv background. The mice were backcrossed twelve generations (N12) to a C57BL/6NTac inbred background. Heterozygotes were intercrossed to generate homozygous targeted mutation mice. The line is maintained by incrossing homozygous mice.

Genetics

Species:Mouse
Strain Type:Congenic
Allele Type:Constitutive Knockout
Coat Color:Black
Genetic Background:C57BL/6

Other Genetic Notes:

Wild type for Nnt mutation

Guides & Publications

Initial Publication: 

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.

Applications & Therapeutic Areas

  • Cell and Tissue Humanized
  • Immunology
  • Inflammation
  • Oncology & Immuno-Oncology
  • Autoimmune Disease
  • Inflammatory Bowel Disease

Transit, Housing & Welfare

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RAGN12 males at 7+ weeks of age are packed as cagemates, without recombination of animals from different cages into a single Taconic Transit Cage (TTC). We recommend that customers do NOT recombine 7+ week old RAGN12 males from different Taconic Transit Cages into the same cage at or after unpacking. 

Packing Practices

Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns.  When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.

Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age."  Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.

Data

Adoptive T cell transfer colitis data in RAGN12 mice

Representative data for adoptive T cell transfer colitis in RAGN12 mice provided by Bolder BioPATH. Download the full data package, including protocol information, colon weight and colon histopathology.
Representative data for adoptive T cell transfer colitis in RAGN12 mice provided by an anonymous pharmaceutical company. Download the full data package.
(Left) Body weights of Rag2 knockout mice following administration of CD4+CD45RBhiCD25- T cells. Mean values are indicated and bars represent SEM; *P<0.05, **P<0.01, ***P<0.001; n=4-6.
(Right) Representative gross colon pathology and splenomegaly of Rag2 knockout mice 60 days following injection with 1 x 106 CD4+CD45RBhiCD25- T cells of saline (control).

Rag2 - Model RAGN12

Conditions of Use for Taconic Transgenic Models™

Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:

  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.

Pricing - USD

Opportunist Free (OF) Health Standard

RAGN12 Female

RAGN12-F Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10$315.00

Specialized Inventory

RAGN12-RF Genotype ko/koQuantity 1 - 199
Retired Female Breeder$315.00

RAGN12 Male

RAGN12-M Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10$315.00

Specialized Inventory

RAGN12-RM Genotype ko/koQuantity 1 - 199
Retired Male Breeder$315.00

Murine Pathogen Free (MPF) Health Standard

RAGN12 Female

RAGN12-F Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10$256.00

Specialized Inventory

RAGN12-RF Genotype ko/koQuantity 1 - 199
Retired Female Breeder$256.00

RAGN12 Male

RAGN12-M Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10$256.00

Specialized Inventory

RAGN12-RM Genotype ko/koQuantity 1 - 199
Retired Male Breeder$256.00

Pricing - EUR

Opportunist Free (OF) Health Standard

RAGN12 Female

RAGN12-F Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10€295,00

Specialized Inventory

RAGN12-RF Genotype ko/koQuantity 1 - 199
Retired Female Breeder€295,00

RAGN12 Male

RAGN12-M Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10€295,00

Specialized Inventory

RAGN12-RM Genotype ko/koQuantity 1 - 199
Retired Male Breeder€295,00

Murine Pathogen Free (MPF) Health Standard

RAGN12 Female

RAGN12-F Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10€238,00

Specialized Inventory

RAGN12-RF Genotype ko/koQuantity 1 - 199
Retired Female Breeder€238,00

RAGN12 Male

RAGN12-M Genotype ko/ko

Age in WeeksQuantity 1 - 999
3 to 10€238,00

Specialized Inventory

RAGN12-RM Genotype ko/koQuantity 1 - 199
Retired Male Breeder€238,00

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