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Rag2 - Model 461
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Overview
Nomenclature: B6.SJL(129S6)-Ptprca/BoyCrTac-Rag2tm1Fwa N10
- Contains a disruption of the recombination activating gene 2 (Rag2)
- Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
- Useful for vaccine development, transplantation studies and hematopoiesis research.
- The Ptprca marker on hematopoietic cells of this congenic strain allows for their identification in immunological adoptive transfer experiments.
- Similar to C57BL/6 with the H2b haplotype but carries the Ptprca and Pep3b genes from the SJL strain.
Origin
The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. Ellis Reinherz of the Dana Farber Cancer Institute received Rag2 mice from the Alt lab on a mixed background. The mice were then backcrossed by S. Koyasu for 10 generations (N10) to B6.SJL-Ptprcα (from Cancer Research). Taconic received stock in 1996. The mice were derived by embryo transfer, and the colony was maintained by mating homozygous mice.
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 12 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
Taconic’s Colony Management experts can design a plan to grow your colony faster.
Genetics
Guides & Publications
Initial Publication:
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.
Applications & Therapeutic Areas
- Immunology
- Oncology & Immuno-Oncology
- Inflammation
Transit, Housing & Welfare
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Rag2 - Model 461
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.
This model is sold under terms which grant perpetual use rights.
This model is sold under terms which grant perpetual use rights.
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