Rag2/Il2rg Double Knockout

Constitutive Knockout

Rag2/II2rg Double Constitutive Knockout Mouse Model

C57BL/6NTac Background

  • Model #
  • Genotype
  • Nomenclature
  • 4111-F
    C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl
  • 4111-M
    C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl
  • Contains a disruption of the recombination activating gene 2 (Rag2).
  • Il2rg deletion ablates functional receptors for many cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15.
  • Mice which lack Rag2 and Il2rg do not develop functional T, B or NK cells.
  • Analysis of spleen cells showed no detectable NK1.1+ cells, B220+ B cells or Thy1+ T cells. A small number of the 1-3 million cells obtained were CD45 low and probably are cells in the macrophage/monocyte lineage.
  • Useful for transplantation of cells otherwise rejected by NK cells in less immunodeficient models.
  • Useful in combination with wild type C57BL/6NTac mice and Rag2 knockout mice on the C57BL/6NTac background for adoptive transfer and mechanistic experiments to study the role of different immune cells.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:



The Rag2 knockout was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells. The Il2rg knockout was made by Xiqing Cao and Warren Leonard at the National Heart, Lung, and Blood Institute by targeting the Il2rg gene in 129S4/SvJae-derived J1 ES cells. Il2rg knockout founders were backcrossed 8 generations to C57BL/6J. Il2rg knockout mice were crossed to Rag2 knockouts on C57BL/10AiTac and maintained on a mixed B6;B10 background. The line was further backcrossed to C57BL/6NTac. SNP testing shows >99% C57BL/6 background genome.





Initial Publication:

  • Garcia S, DiSanto J, Stockinger B. Following the development of a CD4 T cell response in vivo: from activation to memory formation. Immunity 1999 Aug;11(2):163-71.
  • Greenberg P, Riddell S. Deficient cellular immunity--finding and fixing the defects. Science. 1999 Jul 23;285(5427):546-51. Review.
  • Cao X, Shores EW, Hu-Li J, Anver MR, Kelsall BL, Russell SM, Drago J, Noguchi M, Grinberg A, Bloom ET, et al. Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain; Immunity, 1995 Mar;2(3):223-38.
  • Shinkai Y, Rathbun G, Lam KP, Oltz EM, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall AM, and Alt FW. RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangemen; Cell, 1992 Mar 6;68(5):855-67.

Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.

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