NEW MODEL: Jh mouse on B6 background Learn More

Immunogenic peptide from PSF1 identified as potential new cancer vaccine target

Immunogenic peptide from PSF1 identified as potential new cancer vaccine target Although much of the press for immuno-oncology (IO) has focused on checkpoint inhibitors, researchers are exploring many other types of IO therapeutics. Other types of IO therapies under development include bi-specific antibodies, CAR-T cells, and cancer vaccines.

Researchers at Shionogi and Osaka University recently published a paper1 describing identification of a new tumor antigen with the potential for targeting via a cancer vaccine. Previous research showed that PSF1 is expressed in both immature cells as well as cancer stem cells. In this study, Yoshida et al. identified the peptide epitope PSF179-87 both by in silico methods as well as via an experimental approach using mass spectrometry identification of HLA-A2.1-presented PSF1 peptides in a cell culture system. The identified epitope elicited PSF1-specific CD8+ T cell responses in vivo in HLA-A2.1 transgenic mice from Taconic Biosciences. Finally, PSF1 epitope-restricted cytotoxic T lymphocytes (CTLs) were generated from human peripheral blood mononuclear cells (PBMCs) from healthy HLA-A2.1+ donors. These human CTLs demonstrated cytotoxic activity against epitope-pulsed target cells, demonstrating potential utility in the clinic.

Transgenic human HLA mice represent important tools in confirming in vivo immunogenicity of T cell epitopes identified using in silico or in vitro methods. Taconic's portfolio of six different class I HLA transgenics offers coverage for>99% of the world's population. While the original applications for these research tools focused primarily on vaccine development for infectious diseases, a significant increase in use of transgenic HLA mice for cancer immunotherapy applications has been seen.

1. Yoshida, M.; Ishioka, Y.; Ozawa, T.; Okuyama, H.; Iguchi, M.; Ota, T.; Ito, T.; Nagira, M.; Morita, A.; Tanaka, H.; Naito, H.; Kidoya, H.; Takakura, N. Scientific Reports 2017, 7, 11137.