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Carries a transgene consisting of fragments of the human HLA-A*0201 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-A2.1 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains
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Expresses the chimeric HLA-A2.1 class I molecule on the surface of T and B cells
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Represents the human HLA-A2 supertype
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Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology and autoimmune disorders
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Permits identification of epitopes restricted to the HLA-A2 supertype
- Taconic offers this allele on two different backgrounds, CB6F1 and C57BL/6. Model #9659 is on a CB6F1 background and is recommended for most applications, including vaccine research and general epitope identification. The HLA-A2.1 (C57BL/6), model #8906, is recommended specifically for syngeneic tumor studies utilizing C57BL/6-derived tumors and for crossbreeding to other GEM lines on the C57BL/6 background (inquire regarding crossbreeding rights).
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background:
CB6F1 Background Origin:
The HLA-A2.1 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the genomic clone of the human HLA-A*0201 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains. This transgene was microinjected into C57BL/6J zygotes. The resultant mice from Founder Line A*0201 were on an inbred C57BL/6J background. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived, and the colony is maintained through breeding of homozygous males and females. In the production colony, wild type BALB/cAnNTac females are bred to homozygous males on the C57BL/6J background to produce CB6F1 pups, which are sold under model #9659. Color:
Black Agouti Species:
Mouse Initial Publication:
Vitiello A, Marchesini D, Furze J, Sherman LA, Chesnut RW. (1991) Analysis of the HLA-restricted influenza-specific cytotoxic T lymphocyte response in transgenic mice carrying a chimeric human-mouse class I major histocompatibility complex.
J Exp Med 173(4):1007-15.
* This reference describes the transgene incorporated into line 9659. Pharmexa-Epimmune remade the line later directly onto B6/J zygotes. This is the mouse line that Taconic offers as Model # 9659.