Application Areas:
CETP-ApoB100
| Model No. | Nomenclature | Genotype |
|---|---|---|
| 3716-F | B6.SJL-Tg(APOA-CETP)1Dsg Tg(APOB)1102Sgy N10 | tg/wt;tg/wt |
| 3716-M | B6.SJL-Tg(APOA-CETP)1Dsg Tg(APOB)1102Sgy N10 | tg/wt;tg/wt |
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- Diet
Overview
Nomenclature: B6.SJL-Tg(APOA-CETP)1Dsg Tg(APOB)1102Sgy N10
- This double transgenic model carries both human cholesteryl ester transfer protein and human apolipoprotein B100 transgenes
- Exhibits human-like serum HDL/LDL distribution
- When fed a high fat, high cholesterol diet these mice develop foam cell accumulation underlying the endothelium and within the media, and have significantly more complex atherosclerotic lesions than control animals
- Useful for evaluating compounds to treat hypercholesterolemia or HDL/LDL cholesterol imbalance to reduce the risk of developing atherosclerosis
- Differs from model 1007 in that the CETP line used to generate this model has been backcrossed for an additional 6 generations to C57BL/6, thereby reducing genes from the SJL strain
Recommended Controls
The recommended control for this model is C57BL/6NTac.
Origin
The CETP-ApoB100 microinjected model was developed in the laboratory of David Grass at Xenogen Biosciences, now Taconic, by crossing the ApoB100 microinjected model with the CETP microinjected model. At Taconic, the CETP-ApoB100 model is produced by mating the hemizygous ApoB100 transgenic model (1004) with the homozygous CETP transgenic model (3715).
Genetics
Guides & Publications
Initial Publication:
Grass DS, Saini U, Felkner RH, Wallace RE, Lago WJP, Young SG, Swanson ME. (1995) Transgenic Mice expressing both human apolipoprotein B and human CETP have a lipoprotein cholesterol distribution similar to that of normolipidemic humans. J Lipid Res, 36(5): 1082-1090.
Applications & Therapeutic Areas
- Cardiovascular Disease
- Metabolic Disease
Transit, Housing & Welfare
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Packing Practices
Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns. When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.
Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age." Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.
Taconic discourages other types of custom packing requests as they can have a negative impact on animal welfare. Learn more.
Diet
High fat diet-induced metabolic changes in humanized CETP-apoB100 transgenic mice
| Chow | High fat | |
| Body weight (g) | 33.2 ± 1.0 | 43.0 ± 2.0*** |
| Blood glucose (mg/dL) | 142.8 ± 5.6 | 196.4 ± 10.7** |
| Plasma insulin (µU/mL) | 32.0 ± 4.4 | 208.5 ± 16.6*** |
| HOMA-IR (mM*µU/mL/22.5) | 11.3 ± 2.2 | 106.6 ± 10.4*** |
| Total cholesterol (g/L) | 1.01 ± 0.06 | 1.47 ± 0.14** |
| HDL-c (g/L) | 0.39 ± 0.04 | 0.43 ± 0.05 |
| Non-HDL-c (g/L) | 0.61 ± 0.08 | 1.04 ± 0.10** |
| HDL-c/total cholesterol ratio | 0.40 ± 0.05 | 0.29 ± 0.01* |
| Triglycerides (g/L) | 0.79 ± 0.10 | 0.80 ± 0.07 |
| CETP activity (pmol/h/µL) | 43.3 ± 4.0 | 60.2 ± 3.3** |
Table 1. Effects of high-fat diet (D12492, Research diets) in CETP-apoB100 mice (mean ± SEM, n = 7 per group).
*p < 0.05, **p < 0.01, ***p < 0.001 chow versus high fat
Data from Briand et al., Clin Transl Sci. 2011 Dec;4(6):414-20.
Therapeutic control responses in high-fat fed humanized CETP-apoB100 transgenic mice
| Group | Vehicle (p.o) | Metformin | Vehicle | Sitagliptin |
| Body weight (g) | 44.6 ± 2.1 | 44.9 ± 2.1 | 46.5 ± 2.3 | 33.2 ± 0.9¶ |
| Liver mass (g) | ND | ND | 1.4 ± 0.2 | 1.1 ± 0.1 |
| Epididymal fat mass (g) | ND | ND | 0.98 ± 0.14 | 0.36 ± 0.03§ |
| Food intake (g/day) | ND | ND | 3.8 ± 0.03 | 4.3 ± 0.66 |
| hBA1c (%) | 4.8 ± 0.2 | 4.4 ± 0.1* | 4.4 ± 0.1 | 4.2 ± 0.1‡ |
| Blood glucose (mg/dl) | 194 ± 10 | 158 ± 6† | 191 ± 14 | 117 ± 7¶ |
| Insulin (µU/ml) | 123 ± 24 | 61 ± 13* | 106 ± 29 | 24 ± 3§ |
| HOMA-IR (mM x µU.ml/22.5) | 59 ± 15 | 25 ± 6* | 60 ± 20 | 7 ± 2§ |
| AUC glucose (mg/dl x min) | 37163 ± 3107 | 27811 ± 1627† | 35952 ± 4190 | 18970 ± 1237§ |
| Adiponectin (pg/ml) | 18 ± 1 | 17 ± 1 | 15 ± 3 | 17 ± 1‡ |
| Total cholesterol (g/l) | 3.04 ± 0.15 | 2.73 ± 0.13 | 2.56 ± 0.19 | 2.32 ± 0.14 |
| HDL-cholesterol (g/l) | 1.18 ± 0.10 | 1.26 ± 0.05 | 1.01 ± 0.10 | 1.14 ± 0.06 |
| Triglycerides (g/l) | 1.18 ± 0.09 | 1.21 ± 0.06 | 1.38 ± 0.10 | 1.04 ± 0.07§ |
| CETP activity (pmol/µl/h) | 158 ± 5 | 152 ± 5 | 154 ± 6 | 137 ± 3‡ |
| Hepatic total cholesterol (µg/mg liver) | ND | ND | 6.7 ± 0.3 | 5.3 ± 0.5‡ |
| Faecal cholesterol excretion (µg/day) | 6.2 ± 0.8 | 7.1 ± 0.8 | 7.6 ± 0.9 | 17.7 ± 1.3‡ |
| Faecal bile acids excretion (µM/day) | 30.2 ± 5.7 | 28.5 ± 3.5 | 32.9 ± 5.9 | 33.4 ± 1.8 |
Table 2. Body weight and biochemical parameters in high-fat (D12492, Research Diets) fed CETP-apoB100 mice after 3.5 weeks of treatment.
AUC, area under the curve; HbA1c, glycated haemoglobin; HDL, high density lipoprotein; ND, not determined.
*p < 0.05; †p < 0.01 versus vehicle (p.o.); ‡p < 0.05; §p < 0.01; ¶p < 0.001 versus vehicle
Data from Briand et al., Diabetes Obes Metab. 2012 Jul;14(7):662-5.
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CETP-ApoB100
Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
- Title to these Models and biological materials derived from them remains with Taconic.
- The Models will be used for research purposes only.
- The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
- The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
- Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.
Pricing - USD
Murine Pathogen Free (MPF) Health Standard
3716 Female
3716-F Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | US$457.00 |
3716 Male
3716-M Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | US$457.00 |
Pricing - EUR
Murine Pathogen Free (MPF) Health Standard
3716 Female
3716-F Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | 417,00 € |
3716 Male
3716-M Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | 417,00 € |
Pricing - DKK
Murine Pathogen Free (MPF) Health Standard
3716 Female
3716-F Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | kr.3.099,00 |
3716 Male
3716-M Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | kr.3.099,00 |
Pricing - USD Nonprofit
Murine Pathogen Free (MPF) Health Standard
3716 Female
3716-F Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | US$404.00 |
3716 Male
3716-M Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | US$404.00 |
Pricing - EUR Nonprofit
Murine Pathogen Free (MPF) Health Standard
3716 Female
3716-F Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | 367,00 € |
3716 Male
3716-M Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | 367,00 € |
Pricing - DKK Nonprofit
Murine Pathogen Free (MPF) Health Standard
3716 Female
3716-F Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | kr.2.730,00 |
3716 Male
3716-M Genotype tg/wt;tg/wt
Available now
| Age in Weeks | Quantity 1 - 999 |
|---|---|
| 3 to 10 | kr.2.730,00 |
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