Rag2/OT-II

Constitutive Knock Out/Random Transgenic

Rag2/OT-II Constitutive Knock Out/Random Transgenic Mouse Model
Line 1896 ceased commercial production on February 28th. Please see new line number 11490 for more information.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 1896-F
    ko/ko;tg/wt
    B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn
  • 1896-M
    ko/ko;tg/wt
    B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn
  • Carries a transgene that encodes for a T cell receptor (Valpha2 and Vbeta5) specific for a chicken ovalbumin peptide (323-339) presented by the MHC class II molecule H2-Ab1 (I-Ab)
  • Deficient in the recombination activating gene 2 (Rag2) and therefore does not develop mature T and B cells expressing endogenous T cell receptors
  • Virtually all CD4+ cells express the transgene
  • Useful as a source of homogenous donor CD4+ cells for in vivo adoptive transfer studies to investigate T cell development, memory, activation and tolerance
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:

C57BL/6 Background

Origin:

The OT-II microinjected model was developed by Dr. Francis Carbone of Monash Medical School and Dr. William R. Heath of the Walter and Eliza Hall Institute. The model was created by microinjecting the rearranged Valpha2 and Vbeta5 T cell receptor into B6 x B6.C-H2bm1 blastocysts. Taconic received stock from Keio University in June 2001, and the mice were rederived by backcrossing to C57BL/6NTac. OT-II transgenic mice were crossed with a homozygous C57BL/6NTac Rag2 targeted mutation model (RAGN12-M), and then intercrossed for homozygosity at the Rag2 allele. The model was rederived by embryo transfer and is maintained by mating of mice that are homozygous for Rag2 disruption and heterozygous for the OT-II transgene to mice that are homozygous for the Rag2 disruption and wild type for OT-II.


Genetics:

Due to poor production and high incidences of rectal prolapse in the commercial colony, Taconic has backcrossed the parent line 1896 with line RAGN12 (4 generations), and have replaced line 1896 with a new line designation 11490. In doing so, we brought the TCR transgene onto a more pure C57BL/6NTac background. In combination with the backcross strategy, we updated the line mating format to: ko/ko;tg/tg (male or female) by ko/ko;wt/wt. All line animals are hemizygous for the OT-II T cell receptor transgene. An extensive review of flow cytometry expression and frequency of CD4 T cells for hemizygous and homozygous OT-II mice showed that T cell numbers and TCR expression levels were similar. We expect minimal to no functional difference between the genotypes in typical experiments.

Line 1896 ceased commercial production on February 28th.

Color:

Black

Species:

Mouse

Initial Publication:

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.

Barnden MJ, Allison J, Heath WR, Carbone FR. (1998) Defective TCR expression in transgenic mice constructed using cDNA-based α- and Β-chain genes under the control of heterologous regulatory elements. Immunol Cell Biol 76:34-40.