Contains a disruption of the recombination activating gene 2 (Rag2)
Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
Useful for vaccine development, transplantation studies and hematopoiesis research.
The 129S6 strain was the original strain in which the Rag2 targeted mutation was created
Genetic Background: 129S6 Background
Origin: The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. Taconic received stock from the Alt lab in April 1992 on an inbred 129S6 background. Mice were caesarean derived in August 1992. The background differed from a 129S6 background at two biochemical markers, Gpi1a and Pgm1a. In 2000, the mice were backcrossed three generations (N3) to 129S6 to select for the Gpi1c and Pgm1b markers. The colony is maintained in a Restricted Flora™ IBU™ through matings of homozygous mice.
Live production of this model ceased on 1/15/16. Taconic can maintain a dedicated breeding colony for your use or you can purchase breeder stock to set up a colony at your facility, which will require a limited breeding agreement. In addition, consider model on the B6 background model #RAGN12
or BALB/c version #601
Genetics: Wild type for Nnt mutation; carries mutated Disc1
Color: White-Bellied Agouti
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867