Contains a disruption of the recombination activating gene 2 (Rag2)
Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
Useful for vaccine development, transplantation studies and hematopoiesis research.
The 129S6 strain was the original strain in which the Rag2 targeted mutation was created
Genetic Background: 129S6 Background
Origin: The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. Taconic received stock from the Alt lab in April 1992 on an inbred 129S6 background. Mice were caesarean derived in August 1992. The background differed from a 129S6 background at two biochemical markers, Gpi1a and Pgm1a. In 2000, the mice were backcrossed three generations (N3) to 129S6 to select for the Gpi1c and Pgm1b markers. The colony was maintained through matings of homozygous mice.
This model is cryopreserved and available for cryorecovery. You may wish to consider other models with this same mutation which are available from live colonies, models RAGN12
Genetics: Wild type for Nnt mutation; carries mutated Disc1
Color: White-Bellied Agouti
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867