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Carries a transgene coding for the 695-amino acid isoform of human Alzheimer β-amyloid (Aβ) precursor protein
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Isoform derived from a large Swedish family with early-onset Alzheimer's disease (AD)
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Expresses high concentrations of the mutant Aβ, develops significant amyloid plaques and displays memory deficits
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Transgenic mice 11-13 months of age show a 14-fold increase in Aβ(1-42/43) over those at 2-8 months of age
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Elevated Aβ levels are associated with the development of amyloid deposits in frontal, temporal, and entorhinal cortex, hippocampus, presubiculum, subiculum, and cerebellum, with some mice developing the signature Maltese cross pattern of amyloid deposits seen in human AD
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Memory deficits have been demonstrated in 9-10 month old transgenic mice via altered performance in Y maze and Morris water mazes, correlating with the development of amyloid plaques
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Show a significant microglial response to the amyloid plaques with an increase in both number and area
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Useful to study APP expression, amyloid plaque formation, neuronal decline and memory loss associated AD and to study drugs designed for treatment or prevention of AD
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NOTE: Premature mortality is an expected phenotype of this line, with mortality of >20% anticipated, particularly in males. Please take this into consideration when deciding upon order size to retain the needed experimental group sizes
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Shipping Guidelines: Taconic recommends that customers purchase mice between 10 and 12 weeks of age. Due to a phenotype of sudden death at young ages, Taconic cannot guarantee shipment of mice 9 weeks of age or younger.
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This background does not carry the Pde6brd1 retinal degeneration mutation, but as with all 129 substrains does carry a mutated Disc1 gene.
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Between 7 to 12 weeks of age males become aggressive and begin to fight. Male APPSWE mice are shipped in divided Taconic Transit Cages™ with one animal per section. We highly recommend housing APPSWE male mice one per cage.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background:
129S6 Background Origin:
The APPSWE mouse was developed in the laboratory of Karen Hsiao of the University of Minnesota, in association with the Mayo Clinic. This model was created by microinjecting the human APP695 gene containing the double mutation K670N, M671L into B6SJLF2 zygotes using a hamster prion protein cosmid vector. The resultant mice from Founder Line 2576 were backcrossed sixteen generations (N16) to 129S6. Taconic received stock in September 2003. The mice were derived by embryo transfer and are maintained by backcrossing hemizygous male mice with 129S6/SvEvTac female mice.
Availability:
Between 7 to 12 weeks of age males become aggressive and begin to fight. Male APPSWE mice are shipped in divided Taconic Transit Cages™ with one animal per section. We highly recommend housing APPSWE male mice one per cage. Genetics:
Wild type for Nnt mutation and Pde6brd1 mutation; carries mutated Disc1
Color:
White-Bellied Black Agouti Species:
Mouse Initial Publication:
Hsaio K, Chapman P, Nilsen S, Eckman C, Harigaya Y, Younkin S, Yang F, Cole G. (1996) Correlative memory deficits, Aβ elevation, and amyloid plaques in transgenic mice. Science, 274:99-102.
