About the webinar:
Efficient, tissue-specific delivery of biologics remains a major hurdle in therapeutic development. This challenge is especially acute in the central nervous system (CNS), where the blood–brain barrier (BBB) restricts drug access. TfR1-mediated transcytosis has emerged as a strategy to enhance CNS delivery, yet its brain-wide vascular and parenchymal biodistribution is not well quantified at cellular resolution. Moreover, widespread TfR1 expression in peripheral tissues raises concerns about off-target exposure that remain poorly characterized at the whole-organ level.
This webinar explores the use of the humanized transferrin receptor 1 (hTFRC) mouse model to evaluate the biodistribution of TfR1-targeted biologics at cellular and whole-organ resolution. Using clinically relevant TfR1-targeted biologics and non-TfR-binding controls, the study demonstrates how TfR1 targeting influences vascular and parenchymal distribution throughout the brain while also assessing exposure in peripheral TfR1-expressing tissues.
Whole-organ 3D light-sheet fluorescence microscopy (LSFM), AI-based image analysis, and brain-wide regional mapping provide a detailed view of biologic distribution. Results demonstrate enhanced brain parenchymal penetration of TfR1-targeted biologics compared with non-targeted controls and reveal regional differences in both CNS and peripheral tissue exposure.
Attendees will learn how the hTFRC model can support preclinical evaluation of BBB-targeting strategies and how whole-organ biodistribution analysis can provide greater insight into CNS delivery, regional exposure, and potential peripheral liabilities.
Participants will learn to:
- Evaluate TfR1-mediated delivery across the blood-brain barrier
- Compare CNS distribution of TfR1-targeted and non-targeted biologics
- Assess vascular versus parenchymal brain exposure
- Characterize regional viodistribution across brain and peripheral tissues
- Apply the hTFRC model to BBB-penetrant biologic development
- Use whole-organ imaging to inform CNS delivery and translational study design
