The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 1.4 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exon 2 should result in loss of function of the Rdh16 gene by deleting part of the catalytic domain and by generating a frameshift from exon 1 to exon 3 (premature Stop codon in exon 3).
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Rdh16 gene:
- The Rdh9 gene (ENSMUSG00000056148) is located approx. 18 kb upstream of Rdh16 exon 2 in a tail-to-head orientation.
- The Rdh18 gene (ENSMUSG00000054031) is located approx. 13 kb downstream of Rdh16 exon 2 in a head-to-tail orientation.
Datamining and Design performed in 2009.
Gene Family: Dehydrogenase
Genetic Background: C57BL/6
Gene Symbol: Rdh16
Gene Accession Number: NM_009040.2
Allele Type: KO
Availability: Cryopreserved at Taconic