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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 1.4 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exon 2 should result in loss of function of the Rdh16 gene by deleting part of the catalytic domain and by generating a frameshift from exon 1 to exon 3 (premature Stop codon in exon 3).
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According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Rdh16 gene:
- The Rdh9 gene (ENSMUSG00000056148) is located approx. 18 kb upstream of Rdh16 exon 2 in a tail-to-head orientation.
- The Rdh18 gene (ENSMUSG00000054031) is located approx. 13 kb downstream of Rdh16 exon 2 in a head-to-tail orientation.
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Datamining and Design performed in 2009.
Gene Family:
Dehydrogenase Genetic Background:
C57BL/6 Gene Symbol:
Rdh16 Gene Accession Number:
NM_009040.2 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse
