Prkag3 - Model 10595 - KO

• The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic’s C57BL/6N Tac ES cell line.
  
• Prkag3 exon 1 contains the translation initiation codon.
  
• Exons 1 to 13 have been replaced by the FRT site flanked neomycin resistance gene cassette.
  
• The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
  
• The constitutive KO line has been derived using C57BL/6NTac animals.
  
• Deletion of exons 1 to 13 should result in loss of function of the Prkag3 gene by removing all coding exons.
  
• The remaining recombination sites are located in non-conserved regions of the genome.
  
• Datamining and Design performed in 2010.

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.