Pla2g4 - Model 11269 - TG

• The transgenic construct contains 5´ the 8.5 kb mouse CaM-kinase II alpha chain (mCamk2a) promoter fragment, an 850 bp DNA fragment containing the human b-globin intron 2, and 2247bp ORF of human PLA2G4A cDNA (GenBank Acc# NM_024420), followed by 221bp of SV40 poly A sequence at the 3´ end in a pGL3 vector backbone (Promega).
• A consensus KOZAK sequence has been added upstream of the starter ATG.
• The plasmid backbone was removed and the transgenic construct was microinjected in C57BL/6 mice by pronuclear injection.
• Putative transgenic founders were identified by genotyping and bred for germline transmission with C57BL/6 mice.
• Heterozygous transgenic mice were identified by genotyping.
• Line 11266, C57BL/6-Tg(Camk2a-PLA2G4A)31 Mrl, has the highest expression level of the transgene in the brain.
• Line 11269, C57BL/6-Tg(Camk2a-PLA2G4A)9 Mrl, has the medium expression level of the transgene in the brain.
• Datamining and Design performed in 2009.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

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