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The transgenic construct contains 5´ the 8.5 kb mouse CaM-kinase II alpha chain (mCamk2a) promoter fragment, an 850 bp DNA fragment containing the human b-globin intron 2, and 2247bp ORF of human PLA2G4A cDNA (GenBank Acc# NM_024420), followed by 221bp of SV40 poly A sequence at the 3´ end in a pGL3 vector backbone (Promega).
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A consensus KOZAK sequence has been added upstream of the starter ATG.
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The plasmid backbone was removed and the transgenic construct was microinjected in C57BL/6 mice by pronuclear injection.
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Putative transgenic founders were identified by genotyping and bred for germline transmission with C57BL/6 mice.
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Heterozygous transgenic mice were identified by genotyping.
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Line 11266, C57BL/6-Tg(Camk2a-PLA2G4A)31 Mrl, has the highest expression level of the transgene in the brain.
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Line 11269, C57BL/6-Tg(Camk2a-PLA2G4A)9 Mrl, has the medium expression level of the transgene in the brain.
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Datamining and Design performed in 2009.
Gene Family:
Phospholipase Genetic Background:
C57BL/6 Gene Symbol:
Pla2g4 Gene Accession Number:
NM_008869 Allele Type:
TG Source:
Xenogen Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
phospholipase A2, group IVA (cytosolic, calcium-dependent)