Pla2g4 - Model 11269 - TG

• The transgenic construct contains 5´ the 8.5 kb mouse CaM-kinase II alpha chain (mCamk2a) promoter fragment, an 850 bp DNA fragment containing the human b-globin intron 2, and 2247bp ORF of human PLA2G4A cDNA (GenBank Acc# NM_024420), followed by 221bp of SV40 poly A sequence at the 3´ end in a pGL3 vector backbone (Promega).
• A consensus KOZAK sequence has been added upstream of the starter ATG.
• The plasmid backbone was removed and the transgenic construct was microinjected in C57BL/6 mice by pronuclear injection.
• Putative transgenic founders were identified by genotyping and bred for germline transmission with C57BL/6 mice.
• Heterozygous transgenic mice were identified by genotyping.
• Line 11266, C57BL/6-Tg(Camk2a-PLA2G4A)31 Mrl, has the highest expression level of the transgene in the brain.
• Line 11269, C57BL/6-Tg(Camk2a-PLA2G4A)9 Mrl, has the medium expression level of the transgene in the brain.
• Datamining and Design performed in 2009.

NOTICE: Access to this model is conditioned on the requestor's institution agreeing to the terms and conditions of a Material Transfer Agreement (MTA).

Steps to initiate and place order:

1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
2. Nonprofit Investigator inquires on Taconic website.
3. Taconic will answer questions about the GEM lines and the process.
4. Taconic sends request for MTA.
5. Nonprofit Investigator provides scientific workplan answering 4 basic questions.
6. MTA is issued for Nonprofit Investigator/institution review and signature.
7. MTA is executed by all parties and release of GEM is approved.
8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and shipment costs.
9. Taconic begins cryorecovery upon receipt of PO and delivers GEM line to requestor (See pricing and Deliverables).

It is the requestor's obligation to determine that the GEM meets their research needs.

Learn more about Terms of Use, The Scientific Workplan and Frequently Asked Questions.

Upon execution of the MTA terms of use, requestor needs to provide purchase order (PO) to cover the cryorecovery ($4,900 per cryopreserved GEM line) and additional shipment costs.

1. Taconic will begin standard derivation of cryopreserved material (sperm or embryos) upon receipt of PO from recipient.
2. Taconic will conduct testing using its International Health Monitoring System™ (IHMS™) to ensure that the mice meet a Murine Pathogen Free™ (MPF™) health status.
3. Taconic will genotype the recovered mice prior to shipment.
4. Taconic will provide a health status report, the genotyping results of the recovered animals and genotyping assay information for the requested GEM line.
5. Taconic shall deliver a minimum of two (2) mice heterozygous at the selected gene of interest of a requested GEM line.
6. Taconic will make shipping arrangements for the requested GEM to the Licensed Nonprofit recipient.
7. Nonprofit investigator is responsible for covering the shipment costs.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.