Pcsk9 - Model 10898 - cKO

• The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic’s C57BL/6N Tac ES cell line.
• Pcsk9 exon 1 contains the translation initiation codon.
• Exons 2 and 3 have been flanked by loxP sites including the FRT site flanked neomycin resistance gene cassette.
• The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
• The conditional KO line has been derived using C57BL/6NTac animals.
• The constitutive KO allele can be generated by Cre-mediated recombination.
• Deletion of exons 2 and 3 should result in loss of function of the Pcsk9gene.
• The remaining recombination sites are located in non-conserved regions of the genome.
• Datamining and Design performed in 2010.

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