The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic’s C57BL/6N Tac ES cell line.
Pcsk9 exon 1 contains the translation initiation codon.
Exons 2 and 3 have been flanked by loxP sites including the FRT site flanked neomycin resistance gene cassette.
The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
The conditional KO line has been derived using C57BL/6NTac animals.
The constitutive KO allele can be generated by Cre-mediated recombination.
Deletion of exons 2 and 3 should result in loss of function of the Pcsk9gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2010.
Official Gene Symbol: Pcsk9
Gene Family: Protease
Genetic Background: C57BL/6
Gene Symbol: Pcsk9
Gene Accession Number: NM_153565
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: proprotein convertase subtilisin/kexin type 9