Notch1 - Model 13917 - PM

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
• The targeting strategy allows the generation of a constitutive humanization of a regulatory element of the Notch1 gene.
• The targeting strategy is based on the NCBI transcripts NM_008714.3 (mouse) and NM_017617.3 (human).
• A 10 kb mouse genomic sequence located 5 kb upstream of exon 1 (position on chromosome 2: 26,508,823-26,518,822) will be replaced with its human counterpart (position on chromosome 9: 139,445,239-139,455,238).
• The positive selection marker (Puromycin resistance - PuroR) has been flanked by F3 sites and inserted upstream of the 10 kb region. This cassette also contains a loxP site.
• The humanized allele is obtained after in vitro Flp-mediated removal of the selection markers.
• The remaining recombination site will be located in a non-conserved region of the genome.
• Datamining and Design performed 2013.

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.