Orders by weight:
- Super immunodeficient NOG mouse expressing human GM-CSF and human IL-3 cytokines to support myeloid lineage engraftment.
- Higher overall engraftment levels of human hematopoietic stem cells (HSC) compared to core NOG mouse.
- Higher levels of myeloid cell differentiation following human HSC engraftment compared to core NOG mouse.
- The QC specification for huNOG-EXL mice is ≥25% hCD45+ in peripheral blood, but in most cases huNOG-EXL mice average>40% human cells in blood. Note that the % chimerism is relative to the murine compartment. Taconic is not aware of any instances where specific chimerism levels (above the QC threshold) have impacted utility of the model. Chimerism level does not impact the human cell type distribution.
- Model of human allergy response.
- Applications in research involving cancer, infectious disease, immunology, regenerative medicine and humanization.
- Humanized immune system mice require special housing and husbandry. We recommend reviewing the the comprehensive document Policies, Recommendations, & Resources for Taconic's Humanized Immune System Models and the Video: Care of HIS mice for experimental success prior to ordering.
Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Read the Related Taconic Biosciences' Insight:
Origin: hGM-CSF/hIL3 NOG mice engrafted with human CD34+ hematopoietic stem cells (HSCs) stably develop extensive cell lineages as early as 6 to 8weeks post-injection. Both myeloid and lymphoid lineage cells are present in peripheral blood, bone marrow, thymus and spleen and non-lymphoid tissue including lung and liver. Longer term studies are possible in huNOG-EXL mice, with literature reports of stable engraftment and hematopoiesis for at least 20 weeks post engraftment. While, not all human immune cell types have been exhaustively characterized in these mice, extensive myeloid differentiation including granulocyte differentiation (basophil, neutrophil and mast cells) are evident in blood and tissues. Antigen presenting cells (dendritic cells and macrophages) are also increased in frequency, both in blood and spleen.
Availability: Taconic maintains an inventory of female huNOG-EXL mice engrafted as juveniles and FACS-tested at 10 weeks post-engraftment (WPE). These are available to ship at ~12+ WPE. HLA-A2 + or - status is typically available for most huNOG-EXL lots, but this may be impacted by pandemic-related supply chain constraints. Orders with a specification regarding # of mice per donor are subject to availability and are not guaranteed, as orders may need to be adjusted at time of packing. Please inquire regarding custom parameters. If available, these will incur additional fees.
NOTE: Please contact Taconic prior to your first order for a review of unpacking instructions. Tattoo numbers may repeat between engraftment lots. Individual serial number is determined through examination of the tattoo PLUS the box and section location.
- Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, Heike T, Nakahata T. (2002) NOD/SCID/γ mouse: an excellent recipient mouse model for engraftment of human cells. Blood 100(9):3175-3182.
- Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice. Leuk Res 22(9):837-43.
- Ito R, Takahashi T, Katano I, Kawai K, Kamisako T, Ogura T, Ida-Tanaka M, Suemizu H, Nunomura S, Ra C, Mori A, Aiso S, Ito M. (2013) Establishment of a human allergy model using human IL-3/GM-CSF-transgenic NOG mice. J Immunol.191(6):2890-9.