- Double knockout of the glutathione S-transferase, pi 1 and glutathione S-transferase, pi 2 genes
- The Gstp1/2 double knockout is useful for the in vivo study of a Gstp1/2 (regulator of protein synthesis/degradation) function in tumorigenesis, drug metabolism and toxicity.
Genetic Background: C57BL/6
Origin: The Gstp1/2 double knockout was developed in the laboratory of C. Roland Wolf at the Imperial Cancer Research Fund Molecular Pharmacology Unit, Dundee, United Kingdom. A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout. Founder lines were backcrossed to C57BL/6. Taconic received embryos from CRT in 2015.
Source: Cancer Research Technology (CRT)