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Carries a 12 kb fragment of Glial fibrillary acidic protein (Gfap) promoter, 850 bp human β-globin intron 2, and modified firefly luciferase cDNA (Promega pGL-3).
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Basal expression of the reporter is observed primarily in brain. Basal expression of the reporter in non-treated male and female was observed in ear, paw, and tail, and does not change during treatment. The reporter is inducible following injury to the CNS.
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This model provides for the rapid study of transcriptional regulation of the Gfap gene and indications of possible neural injury.
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The Gfap-luc LPTA® animal model is useful in studying changes in the health of nerve tissue, primarily the CNS, resulting in Gfap gene regulation. Changes such as those caused by physical trauma, chemical treatment, and bacterial infections resulting in injury or changes in pressure within the cerebral spinal fluid are most likely to induce Gfap-luc expression.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background:
FVB Background Origin:
The Gfap-luc mouse was developed by Caliper Life Sciences. The model was created by microinjecting a transgene containing a fragment of the glial fibrillary acidic protein promoter, a human β-globin intron 2 and modified firefly luciferase cDNA from pGL3. This transgene was microinjected into FVB/N zygotes. The resultant mice from founder line 53 were bred to FVB/NTac mice and intracrossed to homozygosity. Taconic received stock from Caliper in 2010. The line was embryo transfer derived. The line was maintained by mating homozygous male and female mice.
Color:
Albino Species:
Mouse Initial Publication:
Zhu L, Ramboz S, Hewitt D, Boring L, Grass DS, Purchio AF. (2004) Non-invasive imaging of GFAP expression after neuronal damage in mice. Neurosci Lett. 367(2):210-2.
Other References:
- Luo J, Ho PP, Buckwalter MS, Hsu T, Lee LY, Zhang H, Kim DK, Kim SJ, Gambhir SS, Steinman L, Wyss-Coray T. (2007) Glia-dependent TGF-beta signaling, acting independently of the TH17 pathway, is critical for initiation of murine autoimmune encephalomyelitis. J Clin Invest. 117(11):3306-15.
- Luo J, Ho P, Steinman L, Wyss-Coray T. (2008) Bioluminescence in vivo imaging of autoimmune encephalomyelitis predicts disease. J Neuroinflammation. 5:6.
- Cordeau P Jr, Lalancette-Hébert M, Weng YC, Kriz J. (2008) Live imaging of neuroinflammation reveals sex and estrogen effects on astrocyte response to ischemic injury. Stroke. 39(3):935-42.
- Keller AF, Gravel M, Kriz J.(2009) Live imaging of amyotrophic lateral sclerosis pathogenesis: disease onset is characterized by marked induction of GFAP in Schwann cells. Glia. 57(10):1130-42.
- Tamgüney G, Francis KP, Giles K, Lemus A, DeArmond SJ, Prusiner SB. (2009) Measuring prions by bioluminescence imaging. Proc Natl Acad Sci USA. 106(35):15002-6.
- Lee J, Borboa AK, Baird A, Eliceiri BP. (2011) Non-invasive quantification of brain tumor-induced astrogliosis. BMC Neurosci. 12:9.
- Watts JC, Giles K, Grillo SK, Lemus A, DeArmond SJ, Prusiner SB. (2011) Bioluminescence imaging of Abeta deposition in bigenic mouse models of Alzheimer's disease. Proc Natl Acad Sci USA. 108(6):2528-33.
