The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
The N265S point mutation was introduced into Gabrb2 exon 8.
The neomycin resistance cassette, which is flanked by loxP sites, was introduced into intron 7.
Chimeric mice were produced and germ line transmission achieved.
The neomycin resistance gene has been removed in the constitutive PM mice by crossing to Cre-Deleter mice.
The N265S point mutation in exon 8 abolishes etomidate binding of the Gabrb2 N265S receptor, but keeps the Gabrb2 function otherwise normal.
Gabrb2 N265S constitutive PM mice are in a mixed C57BL/6-129SvEv genetic background.
See D.S. Reynolds et al, J Neurosci. 2003 Sep 17;23(24):8608-17, for further details.
Gene Family: Receptor
Genetic Background: B6:129S6
Gene Symbol: Gabrb2
Gene Accession Number: NM_008070
Allele Type: PM
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: gamma-aminobutyric acid (GABA) A receptor, subunit beta 2