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The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
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The N265S point mutation was introduced into Gabrb2 exon 8.
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The neomycin resistance cassette, which is flanked by loxP sites, was introduced into intron 7.
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Chimeric mice were produced and germ line transmission achieved.
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The neomycin resistance gene has been removed in the constitutive PM mice by crossing to Cre-Deleter mice.
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The N265S point mutation in exon 8 abolishes etomidate binding of the Gabrb2 N265S receptor, but keeps the Gabrb2 function otherwise normal.
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Gabrb2 N265S constitutive PM mice are in a mixed C57BL/6-129SvEv genetic background.
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See D.S. Reynolds et al, J Neurosci. 2003 Sep 17;23(24):8608-17, for further details.
Gene Family:
Receptor Genetic Background:
B6:129S6 Gene Symbol:
Gabrb2 Gene Accession Number:
NM_008070 Allele Type:
PM Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
gamma-aminobutyric acid (GABA) A receptor, subunit beta 2
