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Ubiquitously active general Flp deleter.
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In vitro-derived Flp recombinase under the control of the chicken beta-actin promoter and an hCMV immediate early enhancer. The use of the chimeric CMV enhancer/beta-actin promoter leads to a ubiquitous expression profile in eukaryotes. The Flp recombinase carries a minimal, nuclear localization signal from the SV40 larger T-antigen. Additional elements of the expression cassette are an intron with splice donor and acceptor sites upstream of the Flp gene and a synthetic polyadenylation signal downstream of the Flp.
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This colony is currently being intracrossed to homozygosity. In the interim, mice may be sold as carriers. Mice with a genotype of Carrier(C) carry the transgene, but may be either hemizygous or homozygous.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background:
C57BL/6 Background Origin:
The Flp Deleter mouse was developed by Artemis Pharmaceuticals (now Taconic). The mutation was introduced through random integration (microinjection) of the CAGGS-Flpe into C57BL/6 zygotes. The line was backcrossed 10 generations to C57BL/6NTac and intercrossed to homozygosity. The line was rederived in 2013 and is currently being intracrossed to homozygosity. Color:
Black Species:
Mouse
