Flp Deleter

Random Transgenic

Flp Deleter Random Transgenic Mouse Model

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 7089-F
    tg/wt
    C57BL/6-Tg(CAG-Flpe)2Arte
  • 7089-M
    tg/wt
    C57BL/6-Tg(CAG-Flpe)2Arte
  • 7089-F
    tg/tg
    C57BL/6-Tg(CAG-Flpe)2Arte
  • 7089-M
    tg/tg
    C57BL/6-Tg(CAG-Flpe)2Arte
  • Ubiquitously active general Flp deleter.
  • In vitro-derived Flp recombinase under the control of the chicken beta-actin promoter and an hCMV immediate early enhancer. The use of the chimeric CMV enhancer/beta-actin promoter leads to a ubiquitous expression profile in eukaryotes. The Flp recombinase carries a minimal, nuclear localization signal from the SV40 larger T-antigen. Additional elements of the expression cassette are an intron with splice donor and acceptor sites upstream of the Flp gene and a synthetic polyadenylation signal downstream of the Flp.
  • This colony is currently being intracrossed to homozygosity. In the interim, mice may be sold as carriers. Mice with a genotype of Carrier(C) carry the transgene, but may be either hemizygous or homozygous.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:

C57BL/6 Background

Origin:

The Flp Deleter mouse was developed by Artemis Pharmaceuticals (now Taconic). The mutation was introduced through random integration (microinjection) of the CAGGS-Flpe into C57BL/6 zygotes. The line was backcrossed 10 generations to C57BL/6NTac and intercrossed to homozygosity. The line was rederived in 2013 and is currently being intracrossed to homozygosity.

Color:

Black

Species:

Mouse


Access to this model requires execution of a Flp Deleter Crossbreeding Agreement. There are two types of agreements, a one-time license which permits a single crossbreeding event to a specific mouse line or a broad license which permits crossbreeding to an unlimited number of lines during the term.

The fees for a Flp Deleter Crossbreeding Agreement that permits crossing of the Flp Deleter to ONE (1) specified frted line is:
  • $500/€425 per line for non-profit users
  • $1,500/€1275 per line for for-profit users
The fees for a Flp Deleter Crossbreeding Agreement that permits crossing of the Flp Deleter to an unlimited number of lines during the term is:
  • $4,000/€3400 per year for non-profit users
  • $10,000/€8500 peryear for for-profit users
Once the agreement is executed, you may order mice for use in crossbreeding. Please contact Taconic Customer Service for details.