Floxed p53 Mouse

Conditional Knock Out

Floxed p53 Conditional Knock Out Mouse Model
EZcohort™ Models
The easiest way to acquire and breed cryopreserved GEMs
LEARN MORE or INQUIRE

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 11512-F
    fl/fl
    B6.129P2-Trp53tm2Brn/A
  • 11512-M
    fl/fl
    B6.129P2-Trp53tm2Brn/A
  • Contains a targeted mutation of Trp53 which introduced LoxP sites flanking exons 2 through 10.
  • Cross with the tissue-specific Cre of your choice to generate a conditional disruption of the Trp53 tumor suppressor gene, the most commonly mutated gene in human cancers.
  • Useful for studying Trp53 gene function or screening potential cancer intervention therapies.
  • Conditional mutation avoids the predominance of non-epithelial tumors observed in constitutive Trp53 knockouts.
  • After deletion of the gene via crossing to a tissue-specific Cre line, the incidence and the spectrum of tumors observed in homozygous or heterozygous mutant animals were comparable to those found in constitutive knockouts.

Genetic Background:

C57BL/6 Background

Origin:

The Floxed p53 Mouse was developed in the laboratory of Dr. Anton Berns of the Netherlands Cancer Institute (NKI). The Trp53 mutation was generated by insertion of loxP sites flanking exons 2 through 10. IB10/E15B10 (129P2) embryonic stem cells were targeted and the resulting chimeras were backcrossed to C57BL/6. Taconic received stock in 2011. The line was derived by embryotransfer and backcrossed five generations (N5) to C57BL/6NTac . The line was maintained by incrossing mice homozygous for the floxed allele.


Color:

Black

Species:

Mouse

Initial Publication:

Jonkers J, Meuwissen R, van der Gulden H, Peterse H, van der Valk M, Berns A. (2001) Synergistic tumor suppressor activity of BRCA2 and p53 in a conditional mouse model for breast cancer. Nat Genet. 29(4):418-25.



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.