Contains a targeted mutation of Trp53 which introduced LoxP sites flanking exons 2 through 10.
Cross with the tissue-specific Cre of your choice to generate a conditional disruption of the Trp53 tumor suppressor gene, the most commonly mutated gene in human cancers.
Useful for studying Trp53 gene function or screening potential cancer intervention therapies.
Conditional mutation avoids the predominance of non-epithelial tumors observed in constitutive Trp53 knockouts.
After deletion of the gene via crossing to a tissue-specific Cre line, the incidence and the spectrum of tumors observed in homozygous or heterozygous mutant animals were comparable to those found in constitutive knockouts.
Genetic Background: C57BL/6 Background
The Floxed p53 Mouse was developed in the laboratory of Dr. Anton Berns of the Netherlands Cancer Institute (NKI). The Trp53 mutation was generated by insertion of loxP sites flanking exons 2 through 10. IB10/E15B10 (129P2) embryonic stem cells were targeted and the resulting chimeras were backcrossed to C57BL/6. Taconic received stock in 2011. The line was derived by embryotransfer and backcrossed five generations (N5) to C57BL/6NTac . The line was maintained by incrossing mice homozygous for the floxed allele.
Jonkers J, Meuwissen R, van der Gulden H, Peterse H, van der Valk M, Berns A. (2001) Synergistic tumor suppressor activity of BRCA2 and p53 in a conditional mouse model for breast cancer. Nat Genet. 29(4):418-25.