- May be used to generate constitutive or conditional knockouts of the Jagged 1 gene by crossing to the cre line of choice.
- The conditional Jag1 knockout is useful for in vivo studies focused on tissue specific Jag1 knockouts.
- This line can also be used to study Notch signaling.
Genetic Background: 129;C57BL/6
The Jagged 1 conditional knockout was developed in the laboratory of Julian Lewis at the London Research Institute. A Jagged 1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background. Taconic received embryos from CRT in 2015.
Source: Cancer Research Technology (CRT)
Brooker et al. 2006. Notch ligands with contrasting functions: Jagged1 and Delta1 in the mouse inner ear. Development. 133:1277-86