Cardiovascular Disease:
Bleeding Time
Bleeding time is an in vivo assay indicative of platelet and coagulation function. The bleeding time assay determines whether a particular knockout line has any deficiency with respect to platelet function or coagulation. Mice are anesthetized with isoflurane and then 7 mm of their tail is transected completely. The tail is placed into 37°C saline and the time to bleeding cessation is measured. The maximum time allowed is 5 minutes. At that point the tail is cauterized and the mouse is recovered.
Data are presented in table format:
| Genotype | 0-1 Min | 1-2 Mins | 2-3 Mins | 3-4 Mins | 4-5 Mins | 5+ Mins | Ratio Long Bleed | % Long Bleed |
|---|---|---|---|---|---|---|---|---|
| Homozygous | 1 | 2 | 2 | 0 | 0 | 1 | 1/6 | 17% |
| Wild Type | 1 | 2 | 2 | 0 | 1 | 2 | 2/6 | 25% |
| Heterozygous | 0 | 0 | 0 | 0 | 0 | 0 | 0/0 | 0 |