Nomenclature: C57BL/6-Gt(ROSA)26Sor^{tm1 mrl}

• The targeting vector has been transfected into the TaconicArtemis C57BL/6NTac ES cell line.
• The following elements have been inserted into the ROSA26 locus:
  
  
  • A human Involucrin promoter cassette (IVL).
  • The Slc27a4 open reading frame (based on NCBI transcript NM_011989_2).
  • A synthetic polyadenylation signal.
  • A Neomycin resistance (NeoR) cassette containing a polyadenylation signal and lacking a translation initiation codon.
• 
  
• The constitutive TTG line has been generated by crossing chimeras to C57BL/6NTac animals.
• The SLC27A4 protein is expressed under the control of the Involucrin promoter.
• The presence of the NeoR cassette terminates the transcription from the ROSA26 promoter.
• Datamining and Design performed in 2008.

Availability