Rag2 - Model 578

Rag2  (Model 578) Constitutive Knockout

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Model No.NomenclatureGenotype
578B10.Cg-Rag2tm1Fwa N13Inquire for genotype
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Overview

Nomenclature: B10.Cg-Rag2tm1Fwa N13

  • Contains a disruption of the recombination activating gene 2 (Rag2)
  • Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
  • Useful for vaccine development, transplantation studies and hematopoiesis research.

Origin

The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. NIAID received Rag2 mice from the Alt lab and backcrossed 15 generations to B10.D2 and then further backcrossed for five generations to C57BL/10.

This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 12 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.

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Genetics

Species:Mouse
Strain Type:Congenic
Allele Type:Constitutive Knockout
Genetic Background:C57BL/10

Guides & Publications

Initial Publication: Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.

Transit, Housing & Welfare

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Rag2 - Model 578

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.
This model is sold under terms which grant perpetual use rights.

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