The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3 to 5 have been flanked by loxP sites (size of loxP-flanked region: 1.9 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 3 to 5 should result in loss of function of the Wee1 gene and generates a frame shift.
Datamining and Design performed in 2004.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Wee1
Gene Accession Number: NM_009516
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: WEE 1 homolog 1 (S. pombe)