The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Tspo exon 2 contains the translation initiation codon.
Exons 2-3 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exons 2-3 should result in loss of function of the Tspo gene as no remaining protein is expressed.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2006.
Gene Family: Receptor
Genetic Background: C57BL/6
Gene Symbol: Tspo
Gene Accession Number: NM_009775
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: translocator protein