- The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic's C57BL/6N Tac ES cell line.
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Tnfrsf18 exon 1 contains the translation initiation codon.
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Exons 1 to 5 have been replaced by the FRT site flanked neomycin resistance gene cassette.
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The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
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The constitutive KO line has been derived using C57BL/6NTac animals.
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Bud31 (Edg2) constitutive KO mice are in a mixed C57BL/6-129SvEv genetic backgroundDeletion of exons 1 to 5 should result in loss of function of the Tnfrsf18 gene.
- The remaining recombination sites are located in non-conserved regions of the genome.
- Datamining and Design performed in 2004.
Gene Family:
Receptor Genetic Background:
C57BL/6 Gene Symbol:
Tnfrsf18 Gene Accession Number:
NM_009400 Allele Type:
KO Source:
Xenogen Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
tumor necrosis factor receptor superfamily, member 18