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Tbk1 - Model 11131 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 11131
    C57BL/6-Tbk1tm1.1 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 2 contains the translation initiation codon.
  • Exons 5 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 5 and 6 should result in loss of function of the Tbk1 gene by deleting part of the catalytic domain and by generating a frameshift from exon 4 to exons 7 to 9 (premature Stop codon in exon 7).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • According to NCBI and Ensembl databases, the Xpot gene (ENSMUSG00000034667; EntrezGene 73692) is located approx. 15 kb upstream of Tbk1 exon 5, in tail-to-head orientation.
  • Datamining and Design performed in 2010.

Gene Family:

Kinase

Genetic Background:

C57BL/6

Gene Symbol:

Tbk1

Gene Accession Number:

NM_019786.3

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

TANK-binding kinase 1

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.