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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exon 4 has been flanked by loxP sites (size of loxP-flanked region: 1.1 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exon 4 should result in loss of function of the Sort1 gene by generating a frameshift from exon 3 to exons 5 and 6 (premature Stop codon in exon 5).
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The remaining recombination sites are located in non-conserved regions of the genome.
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The corresponding conditional KO allele is available as line 10972.
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Datamining and Design performed in 2009.
Gene Family:
Sortilin Genetic Background:
C57BL/6 Gene Symbol:
Sort1 Gene Accession Number:
NM_019972.2 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
sortilin 1
