The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Sod2 exon 1 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 3 should result in loss of function of the Sod2 gene by leading to inactivation of the enzyme.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2006.
Gene Family: Dismutase
Genetic Background: C57BL/6
Gene Symbol: Sod2
Gene Accession Number: NM_013671
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: superoxide dismutase 2, mitochondrial