The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 6 to 10 have been flanked by loxP sites (size of loxP-flanked region: 2.9 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of the exons 6 to 10 should result in the loss of function of the Soat2 gene by deleting the transmembrane domains TM2 to TM5, and by generating a frameshift from exon 5 to all downstream exons (premature Stop codon in exon 12).
According to NCBI and Ensembl databases, the Igfbp6 gene is located upstream of the Soat2 gene in tail-to-head orientation.
The corresponding constitutive KO allele is available as line 11355.
Datamining and Design performed in 2010.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Soat2
Gene Accession Number: NM_146064.1
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: sterol O-acyltransferase 2