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The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 6 to 10 have been flanked by loxP sites (size of loxP-flanked region: 2.9 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of the exons 6 to 10 should result in the loss of function of the Soat2 gene by deleting the transmembrane domains TM2 to TM5, and by generating a frameshift from exon 5 to all downstream exons (premature Stop codon in exon 12).
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According to NCBI and Ensembl databases, the Igfbp6 gene is located upstream of the Soat2 gene in tail-to-head orientation.
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The corresponding constitutive KO allele is available as line 11355.
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Datamining and Design performed in 2010.
Gene Family:
Transferase Genetic Background:
C57BL/6 Gene Symbol:
Soat2 Gene Accession Number:
NM_146064.1 Allele Type:
cKO Source:
Taconic Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
sterol O-acyltransferase 2
