The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 14 has been flanked by loxP sites (size of loxP-flanked region: 1 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Removal of exon 14 should result in loss of function of the Smurf2 gene, by removing the C-terminal HECT-domain and creating a frameshift from exon 13 to exons 15 and 16.
Datamining and Design performed in 2008.
Gene Family: Ligase
Genetic Background: C57BL/6
Gene Symbol: Smurf2
Gene Accession Number: NM_025481.1
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: SMAD specific E3 ubiquitin protein ligase 2