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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exon 14 has been flanked by loxP sites (size of loxP-flanked region: 1 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Removal of exon 14 should result in loss of function of the Smurf2 gene, by removing the C-terminal HECT-domain and creating a frameshift from exon 13 to exons 15 and 16.
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Datamining and Design performed in 2008.
Gene Family:
Ligase Genetic Background:
C57BL/6 Gene Symbol:
Smurf2 Gene Accession Number:
NM_025481.1 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
SMAD specific E3 ubiquitin protein ligase 2
