The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3 to 4 have been flanked by loxP sites (size of loxP-flanked region: 2.8 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 3 to 4 should result in loss of function of the Slc27a5 gene by removing half of the AMP-dependant synthetase and ligase domain and by generating a frameshift into downstream exons which generates a premature Stop codon.
The corresponding constitutive KO allele is available as line 9637.
Datamining and Design performed in 2008.
Gene Family: Solute Carrier
Genetic Background: C57BL/6
Gene Symbol: Slc27a5
Gene Accession Number: NM_009512.1
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: solute carrier family 27 (fatty acid transporter), member 5