The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Sgk1 exon 1 contains the translation initiation codon.
Exons 4-11 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exons 4-11 should result in loss of function of the Sgk1 gene by deleting the S/T protein kinase domain.
The remaining recombination sites are located in intron regions of the genome.
The constitutive KO line is also available in a mixed C57BL/6 – 129S2 genetic background, line 9375.
Datamining and Design performed in 2005.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Sgk1
Gene Accession Number: NM_001161845
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: serum/glucocorticoid regulated kinase 1