The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Scd1 exon 1 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
Deletion of exon 2 should result in loss of function of the Scd1 gene by creating a frame shift mutation.
The remaining recombination sites are located in intron regions of the genome.
Datamining and design performed in 2009.
Gene Family: Desaturase
Genetic Background: C57BL/6
Gene Symbol: Scd1
Gene Accession Number: NM_011434
Allele Type: cKO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: stearoyl-Coenzyme A desaturase 1