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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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Scd1 exon 1 contains the translation initiation codon.
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Exon 2 has been flanked by loxP sites.
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
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Deletion of exon 2 should result in loss of function of the Scd1 gene by creating a frame shift mutation.
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The remaining recombination sites are located in intron regions of the genome.
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Datamining and design performed in 2009.
Gene Family:
Desaturase Genetic Background:
C57BL/6 Gene Symbol:
Scd1 Gene Accession Number:
NM_011434 Allele Type:
cKO Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
stearoyl-Coenzyme A desaturase 1
