The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Rps6ka1 exon 1 contains the translation initiation codon.
Exons 8-11 have been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exons 8-11 should result in loss of function of the Rps6ka1 gene by deleting the N-terminal Kinase domain.
The remaining recombination sites are located in intron regions of the genome.
The conditional KO allele is also available as line 9510.
Data mining and Design performed in 2005.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Rps6ka1
Gene Accession Number: NM_009097
Allele Type: KO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: ribosomal protein S6 kinase polypeptide 1