The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Rps6ka1 exon 1 contains the translation initiation codon.
Exons 8-11 have been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination, line 9508.
Deletion of exons 8-11 should result in loss of function of the Rps6ka1 gene by generating a frameshift (premature stop codon in exon 12).
The remaining recombination sites are located in intron regions of the genome.
Data mining and Design performed in 2005.
Gene Family: Kinase
Genetic Background: C57BL/6
Gene Symbol: Rps6ka1
Gene Accession Number: NM_009097
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: ribosomal protein S6 kinase polypeptide 1