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Rock2 - Model 12905 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 12905
    C57BL/6-Rock2 tm1.1 Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
  • Rock2 exon 1 contains the translation initiation codon.
  • Exon 3 has been flanked by loxP sites (size of loxP-flanked region 1.6kb).
  • The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • Deletion of exon 3 should result in the loss of function of the Rock2 gene by deleting part of the protein kinase domain and by generating a frameshift from exon 2 to exons 4-6 (premature Stop codon in exon 4).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • Datamining and Design performed in 2012.

Gene Family:

Kinase

Genetic Background:

C57BL/6

Gene Symbol:

Rock2

Gene Accession Number:

NM_009072

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

Rho-associated coiled-coil containing protein kinase 2

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Taconic continues to strive to gain full access to all models for both non-profit and for-profit organizations. However, at this time some models are currently not available to commercial institutions. Please be sure to check back often as more models are made available on a routine basis.

Non-profit Pricing:

Price per Line ($)
$5,500

Non-profit Pricing:

Price per Line (€)
€5,005