Retsat - Model 10312 - KO

Constitutive Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10312
    C57BL/6-Retsattm1.2 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 1 contains the translation initiation codon.
  • Exons 4 to 5 have been flanked by loxP sites (size of loxP-flanked region: 2.3 kb).
  • The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
  • The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
  • Deletion of exons 4-5 should result in loss of function of the Retsat gene by deleting part of the catalytic domain and by generating a frameshift from exon 3 to exons 6-9 (premature Stop codon in exon 6).
  • According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Retsat gene:

    • The Rbed1 gene (Entrez ID: 232089, ENSMUSG00000056698) is located only 4.3 kb upstream of Retsat exon 4 in a head-to-head orientation.
    • The Tgoln1 gene (Entrez ID: 22134, ENSMUSG00000056429) is located 4.6 kb downstream of Retsat exon 5 in a tail-to-tail orientation.

  • Datamining and Design performed in 2009.

Gene Family:


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Cryopreserved at Taconic



Official Gene Name:

Retsat - Model 10312 - KO

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

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