The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 4 to 5 have been flanked by loxP sites (size of loxP-flanked region: 2.3 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 4-5 should result in loss of function of the Retsat gene by deleting part of the catalytic domain and by generating a frameshift from exon 3 to exons 6-9 (premature Stop codon in exon 6).
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Retsat gene:
- The Rbed1 gene (Entrez ID: 232089, ENSMUSG00000056698) is located only 4.3 kb upstream of Retsat exon 4 in a head-to-head orientation.
- The Tgoln1 gene (Entrez ID: 22134, ENSMUSG00000056429) is located 4.6 kb downstream of Retsat exon 5 in a tail-to-tail orientation.
Datamining and Design performed in 2009.
Gene Family: Reductase
Genetic Background: C57BL/6
Gene Symbol: Retsat
Gene Accession Number: NM_026159.4
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: Retsat - Model 10312 - KO