The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Pten exon 2 contains the translation initiation codon.
Exon 5 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 5 should result in loss of function of the Pten gene by generating a frameshift (premature Stop codon in exon 6).
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2004.
Gene Family: Phosphatase
Genetic Background: C57BL/6
Gene Symbol: Pten
Gene Accession Number: NM_008960
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: phosphatase and tensin homolog