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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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Pten exon 2 contains the translation initiation codon.
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Exon 5 has been flanked by loxP sites.
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 5 should result in loss of function of the Pten gene by generating a frameshift (premature Stop codon in exon 6).
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The remaining recombination sites are located in intron regions of the genome.
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Datamining and Design performed in 2004.
Gene Family:
Phosphatase Genetic Background:
C57BL/6 Gene Symbol:
Pten Gene Accession Number:
NM_008960 Allele Type:
cKO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
phosphatase and tensin homolog
